编码人肝脏羧酸酯酶的cDNA的克隆与分析

Cloning and analysis of a cDNA encoding a human liver carboxylesterase.

作者信息

Riddles P W, Richards L J, Bowles M R, Pond S M

机构信息

CSIRO, Division of Tropical Animal Production, Long Pocket Laboratories, Indooroopilly, Queensland, Australia.

出版信息

Gene. 1991 Dec 15;108(2):289-92. doi: 10.1016/0378-1119(91)90448-k.

DOI:10.1016/0378-1119(91)90448-k

PMID:1748313

Abstract

A human liver carboxylesterase (CE)-encoding cDNA has been cloned using synthetic oligodeoxyribonucleotides (oligos) based on the known amino acid (aa) sequences of rabbit and rat liver CEs. The oligos hybridize specifically to DNA encoding liver CEs. The longest cDNA obtained from screening several cDNA libraries encodes about 80% of the protein and translates into an aa sequence which has a high degree of similarity with the sequences of liver CEs from other species. On hybridization to mRNA isolated from human liver, the cDNA gave a single band of about 2.0 kb consistent with its encoding a protein of less than 68 kDa. DNA obtained from a number of human livers and probed with the CE cDNA gave identical hybridization patterns. These patterns were moderately complex by comparison with published data.

摘要

利用基于兔和大鼠肝脏羧酸酯酶（CE）已知氨基酸序列合成的寡聚脱氧核糖核苷酸（寡核苷酸），克隆了人肝脏羧酸酯酶（CE）编码cDNA。这些寡核苷酸与编码肝脏CE的DNA特异性杂交。从筛选多个cDNA文库中获得的最长cDNA编码约80%的蛋白质，并翻译成一个与其他物种肝脏CE序列具有高度相似性的氨基酸序列。与从人肝脏分离的mRNA杂交时，该cDNA产生了一条约2.0 kb的单带，与其编码小于68 kDa的蛋白质一致。从多个肝脏获得的DNA并用CE cDNA进行探针杂交，得到了相同的杂交模式。与已发表的数据相比，这些模式中等复杂。