Sharma V K, Jadhav R K, Rao G J, Saraf A K, Chandra H
Medicolegal Institute, Gandhi Medical College Building, Bhopal, India.
Forensic Sci Int. 1991 Sep;50(2):255-61. doi: 10.1016/0379-0738(91)90156-d.
A method has been developed on reverse phase high performance liquid chromatography for simultaneous determination of methyl, ethyl and isopropyl alcohols under refractive index detection using pure water as the mobile phase. A good separation has been achieved between these alcohols. Detector response was linear with a detection limit of 5 mg/100 ml. Recovery studies were performed by adding known amounts of methyl and ethyl alcohols to blood, lung and liver within the range 80-90%. The reproducibility of the results was always greater than 90%. The quantitative distribution of methyl alcohol in postmortem body tissues and fluids has been reported in three cases of poisoning.
已开发出一种反相高效液相色谱法，以纯水为流动相，在折光率检测下同时测定甲醇、乙醇和异丙醇。这些醇类之间实现了良好的分离。检测器响应呈线性，检测限为5 mg/100 ml。通过向血液、肺和肝脏中添加80 - 90%范围内已知量的甲醇和乙醇进行回收率研究。结果的重现性始终大于90%。报告了3例中毒病例中甲醇在死后尸体组织和体液中的定量分布情况。
