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利用等位酶标记研究濒危特有物种短体霍氏鲶的群体遗传结构

Population genetic structure of endemic and endangered yellow catfish, Horabagrus brachysoma, using allozyme markers.

作者信息

Muneer P M A, Gopalakrishnan A, Lal K K, Mohindra V

机构信息

National Bureau of Fish Genetic Resources, Cochin Unit, CMFRI Campus, Kochi, Kerala, 682 018, India.

出版信息

Biochem Genet. 2007 Oct;45(9-10):637-45. doi: 10.1007/s10528-007-9084-z. Epub 2007 May 5.

Abstract

The allozyme variation and population genetic structure of Horabagrus brachysoma in three natural populations from the southern part of the Western Ghats region, India, were investigated by polyacrylamide gel electrophoresis. Variations at 14 loci from 14 enzyme systems were analyzed. The allozyme analysis revealed a high level of genetic variation in this species, with an average of observed alleles per locus of 2.357 and observed heterozygosity of 0.178. The positive value of the fixation index (FIS=0.507) implied a significant deficiency of heterozygosity at the population level. The highly significant probability (P<0.0001) for the overall loci suggested that the three sample sets were not part of the same gene pool.

摘要

通过聚丙烯酰胺凝胶电泳,对来自印度西高止山脉地区南部三个自然种群的短体霍拉鲇的等位酶变异和群体遗传结构进行了研究。分析了14种酶系统中14个位点的变异情况。等位酶分析显示该物种具有高水平的遗传变异,每个位点观察到的等位基因平均为2.357个,观察到的杂合度为0.178。固定指数的正值(FIS = 0.507)表明在群体水平上杂合子存在显著不足。所有位点的高度显著概率(P < 0.0001)表明这三个样本组并非同一基因库的一部分。

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