Yang J, Mao M, Liu K, Yang G, Sun Z, Lin X
Hua Xi Yi Ke Da Xue Xue Bao. 1991 Sep;22(3):256-8.
A simple method is reported for the isolation of desmosomes. The fresh noncornified layers of cow nose epidermis were immersed in 0.1 mol/L citric acid-sodium citrate buffer (pH 2.3, containing 0.5 mmol/L PMSF), and then treated with homogenization and discontinuous sucrose density gradient centrifugation. Desmosomes were located at the 50-56% sucrose interface. Electron microscopy revealed that the characteristic desmosome structure was well preserved, and that a few intermediate filament bundles attached to desmosome plaque were removed by the solubilizing action of the buffer. Approximate 100 mg desmosomes (dry weight) were got from 20g wet noncornified layers of epidermic tissue.
报道了一种分离桥粒的简单方法。将新鲜的牛鼻表皮非角质化层浸入0.1 mol/L柠檬酸 - 柠檬酸钠缓冲液（pH 2.3，含0.5 mmol/L苯甲基磺酰氟）中，然后进行匀浆处理并通过不连续蔗糖密度梯度离心法进行分离。桥粒位于50 - 56%的蔗糖界面处。电子显微镜观察显示，桥粒的特征结构保存良好，并且通过缓冲液的溶解作用，一些附着在桥粒斑上的中间丝束被去除。从20克湿的非角质化表皮组织层中大约获得了100毫克（干重）的桥粒。
