Ohashi Yuji, Tokunaga Makoto, Taketomo Naoki, Ushida Kazunari
Laboratory of Animal Science, Kyoto Prefectural University, Shimogamo, Kyoto 606-8522, Japan.
J Nutr Sci Vitaminol (Tokyo). 2007 Feb;53(1):82-6. doi: 10.3177/jnsv.53.82.
The aim of this study was to evaluate the effect of feeding yoghurt, prepared with Lactobacillus delbrueckii subsp. bulgaricus strain 2038, on indigenous lactobacilli in the pig cecum. Three female pigs fistulated at the cecum were fed 250 g of this yoghurt that contained over 10(11) colony-forming units of L. delbrueckii subsp. bulgaricus strain 2038 with their daily meal for 2 wk. The relative abundance and the composition of cecal lactobacilli was monitored by analysis of bacterial 16S rDNA with real time PCR and amplified bacterial rDNA restriction analysis using Lactobacillus-group specific primers, respectively, for 2 wk prior to, at the end of 2 wk of and 2 wk after the administration of this yoghurt. The relative abundance of lactobacilli was significantly increased by feeding yoghurt (p<0.01), although the bacterial 16S rDNA matching L. delbrueckii subsp. bulgaricus strain 2038 was not detected by amplified bacterial rDNA restriction analysis during this study. The number of operational taxonomic units (OTUs) detected was increased with feeding of the yoghurt in all pigs. At the same time, the estimated cell number of each OTU was increased with feeding of the yoghurt. It is demonstrated that continuous consumption of the probiotic lactobacilli will stimulate the growth of some indigenous lactobacilli and alter the composition of the lactobacilli.
本研究的目的是评估用德氏乳杆菌保加利亚亚种2038菌株制备的酸奶对猪盲肠内源性乳酸菌的影响。对3头盲肠造瘘的母猪，在它们每日的日粮中添加250克这种含有超过10¹¹个德氏乳杆菌保加利亚亚种2038菌落形成单位的酸奶，持续2周。分别在给予这种酸奶前2周、给予2周结束时以及给予后2周，通过实时PCR分析细菌16S rDNA和使用乳酸菌属特异性引物进行扩增细菌rDNA限制性分析，监测盲肠乳酸菌的相对丰度和组成。尽管在本研究中通过扩增细菌rDNA限制性分析未检测到与德氏乳杆菌保加利亚亚种2038匹配的细菌16S rDNA，但饲喂酸奶后乳酸菌的相对丰度显著增加（p<0.01）。所有猪饲喂酸奶后检测到的可操作分类单元（OTU）数量均增加。同时，每个OTU的估计细胞数也随着酸奶的饲喂而增加。结果表明，持续食用益生菌乳酸菌会刺激一些内源性乳酸菌的生长并改变乳酸菌的组成。
