Igarashi Tomoko, Araki Satohiko, Mori Hidezo, Takeda Soichi
Department of Cardiac Physiology, National Cardiovascular Center Research Institute 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
FEBS Lett. 2007 May 29;581(13):2416-22. doi: 10.1016/j.febslet.2007.04.057. Epub 2007 Apr 30.
Catrocollastatin/vascular apoptosis-inducing protein (VAP)2B is a metalloproteinase from Crotalus atrox venom, possessing metalloproteinase/disintegrin/cysteine-rich (MDC) domains that bear the typical domain architecture of a disintegrin and metalloproteinase (ADAM)/adamalysin/reprolysin family proteins. Here we describe crystal structures of catrocollastatin/VAP2B in three different crystal forms, representing the first reported crystal structures of a member of the monomeric class of this family of proteins. The overall structures show good agreement with both monomers of atypical homodimeric VAP1. Comparison of the six catrocollastatin/VAP2B monomer structures and the structures of VAP1 reveals a dynamic, modular architecture that may be important for the functions of ADAM/adamalysin/reprolysin family proteins.
猫蛇凝血环肽/血管凋亡诱导蛋白（VAP）2B是一种源自西部菱斑响尾蛇毒液的金属蛋白酶，具有金属蛋白酶/去整合素/富含半胱氨酸（MDC）结构域，这些结构域具有去整合素和金属蛋白酶（ADAM）/解聚素/类蛇毒金属蛋白酶家族蛋白的典型结构域架构。在此，我们描述了猫蛇凝血环肽/VAP2B以三种不同晶体形式存在的晶体结构，这是该蛋白家族单体类成员首次报道的晶体结构。整体结构与非典型同二聚体VAP1的两个单体都有很好的一致性。对六个猫蛇凝血环肽/VAP2B单体结构和VAP1结构的比较揭示了一种动态的模块化架构，这可能对ADAM/解聚素/类蛇毒金属蛋白酶家族蛋白的功能很重要。
