Menu P, Labrude P, Grandgeorge M, Vigneron C
Regional Centre for Blood Transfusion and Hematology, Vandoeuvre-les-Nancy, France.
Int J Artif Organs. 1991 Oct;14(10):672-5.
It has only been realized quite recently how important is the purification of hemoglobin solution for use in transfusion and several techniques have been published. We used ion exchange chromatography with which the main "contaminants" (glycoproteins, enzymes, phospholipids) are absorbed by the gel, whereas hemoglobin is not retained. The solutions studied here are non-modified hemoglobin and its homologue pyridoxylated hemoglobin (PLP-Hb). Physico-chemical analyses, usually undertaken to characterize hemoglobin solutions, show no difference before and after purification, except that the enzymatic activity almost disappears. In order to appreciate the benefits of purification, total exchange transfusions were carried out on rats. Without reperfusion, purification of the hemoglobin solution allowed a significantly longer survival time which was even more significant with PLP-Hb solution. Urinary loss did not seem to be affected by purification. With reperfusion in order to compensate these renal losses, PLP-Hb solutions gave survival times up to three days. However, the inevitable death of the animals poses the problem of instability of these purified solutions following enzyme loss.
直到最近人们才意识到，用于输血的血红蛋白溶液的纯化是多么重要，并且已经发表了几种技术。我们使用离子交换色谱法，主要的“污染物”（糖蛋白、酶、磷脂）会被凝胶吸附，而血红蛋白不会被保留。这里研究的溶液是非修饰血红蛋白及其同系物吡哆醛化血红蛋白（PLP-Hb）。通常用于表征血红蛋白溶液的物理化学分析表明，纯化前后没有差异，只是酶活性几乎消失。为了评估纯化的益处，对大鼠进行了全血交换输血。在没有再灌注的情况下，血红蛋白溶液的纯化使存活时间显著延长，对于PLP-Hb溶液来说更是如此。尿液流失似乎不受纯化的影响。为了补偿这些肾脏损失而进行再灌注时，PLP-Hb溶液的存活时间可达三天。然而，动物不可避免的死亡提出了这些纯化溶液在酶损失后稳定性的问题。
