Jaiswal Yogesh Kumar, Chaturvedi Madan Mohan, Deb Kaushik
Molecular Biology and Reproductive Immunology Laboratory, School of Studies in Biochemistry, Jiwaji University, Gwalior, India.
Infect Dis Obstet Gynecol. 2006;2006:32050. doi: 10.1155/IDOG/2006/32050.
Mammalian embryonic development is regulated by several cytokines and growth factors from embryonic or maternal origins. Since CSF-1 plays important role in embryonic development and implantation, we investigated its role in gram-negative bacterial LPS-induced implantation failure. The effect of LPS on normal (nonsuperovulated) and superovulated in vivo-produced embryos was assessed by signs of morphological degeneration. A significantly similar number of morphologically degenerated embryos recovered from both nonsuperovulated and superovulated LPS treated animals on day 2.5 of pregnancy onwards were morphologically and developmentally abnormal as compared to their respective controls (P < .001. Normal CSF-1 expression level and pattern were also altered through the preimplantation period in the mouse embryos and uterine horns after LPS treatment. This deviation from the normal pattern and level of CSF-1 expression in the preimplantation embryos and uterine tissues suggest a role for CSF-1 in LPS-induced implantation failure.
哺乳动物胚胎发育受多种源自胚胎或母体的细胞因子和生长因子调控。由于集落刺激因子-1（CSF-1）在胚胎发育和着床过程中发挥重要作用，我们研究了其在革兰氏阴性菌脂多糖（LPS）诱导的着床失败中的作用。通过形态学退变迹象评估LPS对正常（未超排）和超排体内产生的胚胎的影响。与各自对照组相比，在妊娠第2.5天及之后，从未超排和超排且经LPS处理的动物中回收的形态学退变胚胎数量显著相似，这些胚胎在形态和发育上均异常（P <.001）。LPS处理后，小鼠胚胎和子宫角在植入前阶段CSF-1的正常表达水平和模式也发生了改变。着床前胚胎和子宫组织中CSF-1表达模式和水平偏离正常，提示CSF-1在LPS诱导的着床失败中起作用。
