Kawaguchi Masakazu, Tanabe Hideya, Nagamine Kenichi
Research & Development Division, Research & Development Center, Nichirei Biosciences Inc, Tokyo, Japan.
Biosci Biotechnol Biochem. 2007 May;71(5):1130-5. doi: 10.1271/bbb.60513. Epub 2007 May 7.
The novel flavonoid, leucocyanidin-3-O-beta-D-glucoside, possessing a 4,2''-glycosidic linkage was isolated from green mature acerola (Malpighia emarginata DC.) puree and given the trivial name "aceronidin." To examine the functions of aceronidin, its antioxidative activity and both its alpha-glucosidase and alpha-amylase inhibition activities, as a potential inhibitor of the sugar catabolic enzyme, were evaluated against those of taxifolin, catechin, isoquercitrin and quercitrin which each have a similar structure. The 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical quenching activity of aceronidin was stronger than that of alpha-tocopherol and comparable to that of flavonoids. In the yeast alpha-glucosidase inhibitory assay, aceronidin showed significantly greater inhibition than the other flavonoids tested. In the human salivary alpha-amylase inhibitory assay, aceronidin showed inhibition activity. Taken together, these results indicate aceronidin to be a potent antioxidant that may be valuable as an inhibitor of sugar catabolic enzymes.
从青熟针叶樱桃（Malpighia emarginata DC.）果泥中分离出一种新型黄酮类化合物——无色花青素-3-O-β-D-葡萄糖苷，其具有4,2''-糖苷键，并赋予其俗名“针叶樱桃苷”。为研究针叶樱桃苷的功能，将其作为潜在的糖分解酶抑制剂，针对与它结构相似的花旗松素、儿茶素、异槲皮苷和槲皮苷，评估了其抗氧化活性以及α-葡萄糖苷酶和α-淀粉酶抑制活性。针叶樱桃苷的1,1-二苯基-2-苦基肼（DPPH）自由基淬灭活性强于α-生育酚，与黄酮类化合物相当。在酵母α-葡萄糖苷酶抑制试验中，针叶樱桃苷的抑制作用明显强于其他受试黄酮类化合物。在人唾液α-淀粉酶抑制试验中，针叶樱桃苷表现出抑制活性。综上所述，这些结果表明针叶樱桃苷是一种有效的抗氧化剂，可能作为糖分解酶抑制剂具有重要价值。
