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在5-溴脱氧尿苷诱导GOTO神经母细胞瘤细胞体外分化为施万细胞的过程中，HOXC6和HOXC11可增加S100β基因的转录。

HOXC6 and HOXC11 increase transcription of S100beta gene in BrdU-induced in vitro differentiation of GOTO neuroblastoma cells into Schwannian cells.

作者信息

Zhang Xiuru, Hamada Jun-ichi, Nishimoto Arata, Takahashi Yoko, Murai Taichi, Tada Mitsuhiro, Moriuchi Tetsuya

机构信息

Division of Cancer-Related Genes, Institute for Genetic Medicine, Hokkaido University, Kita-15 Nishi-7, Kita-ku, Sapporo 060-0815, Japan.

出版信息

J Cell Mol Med. 2007 Mar-Apr;11(2):299-306. doi: 10.1111/j.1582-4934.2007.00020.x.

DOI:10.1111/j.1582-4934.2007.00020.x
PMID:17488478
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3822828/
Abstract

HOX genes encode transcription factors that play a key role in morphogenesis and cell differentiation during embryogenesis of animals. Human neuroblastoma cells are known to be chemically induced to differentiate into neuronal or Schwannian cells. In the present study, we investigated the roles of HOX genes in differentiation of GOTO neuroblastoma cells into Schwannian cells. When GOTO cells were grown in the presence of 5-bromo-2'-deoxyuridine (BrdU), they increased the expressions of two HOX genes (HOXC6 and HOXC11) and marker genes for Schwannian cells (S100beta and myelin basic protein). Forced expression of HOXC11 alone or both HOXC6 isoform 1 and HOXC11 induced the expression of S100beta in GOTO cells. In transient transfection experiments, the overexpression of HOXC6 and HOXC11 transactivated the S100beta promoter-reporter construct. Taken together, our results suggest that HOXC6 and HOXC11 are associated with differentiation of GOTO cells into Schwannian cells through the transcriptional activation of S100beta gene.

摘要

HOX基因编码转录因子，这些转录因子在动物胚胎发育过程中的形态发生和细胞分化中起关键作用。已知人类神经母细胞瘤细胞可通过化学诱导分化为神经元细胞或施万细胞。在本研究中，我们调查了HOX基因在GOTO神经母细胞瘤细胞分化为施万细胞过程中的作用。当GOTO细胞在5-溴-2'-脱氧尿苷（BrdU）存在的情况下生长时，它们增加了两个HOX基因（HOXC6和HOXC11）以及施万细胞标记基因（S100β和髓磷脂碱性蛋白）的表达。单独强制表达HOXC11或同时强制表达HOXC6亚型1和HOXC11均可诱导GOTO细胞中S100β的表达。在瞬时转染实验中，HOXC6和HOXC11的过表达激活了S100β启动子-报告基因构建体。综上所述，我们的结果表明，HOXC6和HOXC11通过S100β基因的转录激活与GOTO细胞向施万细胞的分化相关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/e6c36f84455c/jcmm0011-0299-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/02d8977aa857/jcmm0011-0299-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/788da825c1f3/jcmm0011-0299-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/d45cedb91f74/jcmm0011-0299-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/0c1a91d25a19/jcmm0011-0299-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/e6c36f84455c/jcmm0011-0299-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/02d8977aa857/jcmm0011-0299-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/788da825c1f3/jcmm0011-0299-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/d45cedb91f74/jcmm0011-0299-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/0c1a91d25a19/jcmm0011-0299-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7637/3822828/e6c36f84455c/jcmm0011-0299-f5.jpg

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2
Position-specific expression of Hox genes along the gastrointestinal tract.
Congenit Anom (Kyoto). 2004 Mar;44(1):18-26. doi: 10.1111/j.1741-4520.2003.00004.x.
3
Expression profiles of 39 HOX genes in normal human adult organs and anaplastic thyroid cancer cell lines by quantitative real-time RT-PCR system.通过定量实时逆转录聚合酶链反应系统检测39个HOX基因在正常成人器官和间变性甲状腺癌细胞系中的表达谱。
线粒体功能障碍诱导的 H3K27 高乙酰化扰乱帕金森病中的增强子。
JCI Insight. 2021 Sep 8;6(17):e138088. doi: 10.1172/jci.insight.138088.
4
Homeotic Genes: Clustering, Modularity, and Diversity.同源异型基因：聚类、模块化与多样性
Front Cell Dev Biol. 2021 Aug 11;9:718308. doi: 10.3389/fcell.2021.718308. eCollection 2021.
5
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Cancer Manag Res. 2019 Sep 3;11:8167-8179. doi: 10.2147/CMAR.S209904. eCollection 2019.
6
A Risk Classification System With Five-Gene for Survival Prediction of Glioblastoma Patients.一种用于胶质母细胞瘤患者生存预测的五基因风险分类系统。
Front Neurol. 2019 Jul 16;10:745. doi: 10.3389/fneur.2019.00745. eCollection 2019.
7
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J Cachexia Sarcopenia Muscle. 2018 Dec;9(7):1255-1268. doi: 10.1002/jcsm.12363. Epub 2018 Nov 30.
8
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9
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4
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5
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6
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7
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