[系统性红斑狼疮患者外周血中Foxp3 + CD4 + CD25 +调节性细胞的分析]

[Analysis of Foxp3+ CD4+ CD25+ regulatory cells in peripheral blood of patients with SLE].

作者信息

Wei Hong-gang, Cai Bei, Wang Lan-lan, Li Jia, Feng Wei-hua

机构信息

Clinical Immunological Laboratory, West China Hospital of Sichuan University, Chengdu 610041, China.

出版信息

Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2007 May;23(5):432-5.

PMID:17488604

Abstract

AIM

To investigate the expression of GITR on T cells and the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood of patients with SLE.

METHODS

The expression of GITR and Foxp3(+) CD4(+) CD25(+) regulatory T cells in the peripheral blood was determined by flow cytometry(FCM).

RESULTS

Compared with health control, the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells in peripheral blood from SLE patients was lower (P<0.05), but there is no difference in the expression of Foxp3(+) CD4(+) CD25(+) regulatory T cells between active and inactive SLE patients (P>0.05). GITR expression on both CD3(+) CD4(+) T cells and CD3(+) CD8(+) T cells of SLE patients increased significantly (P>0.05). With the development of SLE activity, GITR expression didn't change significantly on both CD3(+) CD4(+) T cells (P>0.05) and CD3(+) CD8(+) T cells (P>0.05).

CONCLUSION

The expression abnormality of both Foxp3(+) CD4(+) CD25(+) regulatory cells and GITR may play important role in the imbalance of immune homeostasis in SLE.

摘要

目的

探讨系统性红斑狼疮（SLE）患者外周血中糖皮质激素诱导肿瘤坏死因子受体（GITR）在T细胞上的表达以及叉头框蛋白3（Foxp3）阳性CD4阳性CD25阳性调节性T细胞的表达情况。

方法

采用流式细胞术（FCM）检测外周血中GITR及Foxp3阳性CD4阳性CD25阳性调节性T细胞的表达。

结果

与健康对照相比，SLE患者外周血中Foxp3阳性CD4阳性CD25阳性调节性T细胞的表达降低（P<0.05），但活动期与非活动期SLE患者之间Foxp3阳性CD4阳性CD25阳性调节性T细胞的表达无差异（P>0.05）。SLE患者CD3阳性CD4阳性T细胞和CD3阳性CD8阳性T细胞上的GITR表达均显著增加（P>0.05）。随着SLE活动度的进展，CD3阳性CD4阳性T细胞（P>0.05）和CD3阳性CD8阳性T细胞（P>0.05）上的GITR表达均无明显变化。