Dutta Moumita, Ghosh Amar N
Division of Electron Microscopy, National Institute of Cholera and Enteric Diseases, Kolkata, India.
Intervirology. 2007;50(4):264-72. doi: 10.1159/000102469. Epub 2007 May 8.
To characterize Vibrio cholerae El Tor typing phage S20 (ATCC No. 51352-B3).
The phage has a hexagonal head and a short tail. Cryo-electron microscopy and three-dimensional image reconstruction showed that the phage head has icosahedral symmetry. The phage has two major structural polypeptides of 50 and 42 kDa. Adsorption of the phage to its host followed a biphasic kinetics and its intracellular growth is characterized by a latent period of 12 min and a burst size of around 60 particles per infected cell. The phage was found to be stable at a pH range 5.0-9.0 and moderately thermotolerant and highly UV sensitive. Phage genome comprises a 40.7 +/- 1.5-kb linear DNA molecule with random circular permutation and terminal redundancy. The restriction endonucleases AccI, HpaII, HaeIII, HindIII, EcoRV, HincII, DraI and XmnI cut vibriophage S20 DNA.
Vibriophage S20, which belongs to Podoviridae, has an icosahedral head and the genome, which is double-stranded linear DNA, has random circular permutation and terminal redundancy.
鉴定霍乱弧菌埃尔托生物型分型噬菌体S20（美国典型培养物保藏中心编号51352 - B3）。
该噬菌体有一个六边形头部和一条短尾。冷冻电子显微镜及三维图像重建显示噬菌体头部具有二十面体对称性。该噬菌体有两条主要结构多肽，分子量分别为50 kDa和42 kDa。噬菌体对宿主的吸附遵循双相动力学，其胞内生长的特征是潜伏期为12分钟，每个感染细胞的爆发量约为60个粒子。发现该噬菌体在pH值5.0 - 9.0范围内稳定，具有中等耐热性且对紫外线高度敏感。噬菌体基因组由一个40.7±1.5 kb的线性DNA分子组成，具有随机环状排列和末端冗余。限制性内切酶AccI、HpaII、HaeIII、HindIII、EcoRV、HincII、Drai和XmnI可切割霍乱弧菌噬菌体S20 DNA。
属于短尾噬菌体科的噬菌体S20具有二十面体头部，其基因组为双链线性DNA，具有随机环状排列和末端冗余。
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