Mondal Payel, Mallick Bani, Dutta Moumita, Dutta Shanta
Division of Electron Microscopy, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
Division of Bacteriology, ICMR-National Institute of Cholera and Enteric Diseases, Kolkata, West Bengal, India.
Front Microbiol. 2022 Aug 22;13:980025. doi: 10.3389/fmicb.2022.980025. eCollection 2022.
is one of the common causal agents of bacterial gastroenteritis-related morbidity and mortality among children below 5 years and the elderly populations. Salmonellosis in humans is caused mainly by consuming contaminated food originating from animals. The genus has several serovars, and many of them are recently reported to be resistant to multiple drugs. Therefore, isolation of lytic bacteriophages in search of bactericidal activity has received importance. In this study, a phage STWB21 was isolated from a lake water sample and found to be a novel lytic phage with promising potential against the host bacteria However, some polyvalence was observed in their broad host range. In addition to the phage STWB21 was able to infect and a few other bacterial species such as and The newly isolated phage STWB21 belongs to the family with an icosahedral head and a long flexible non-contractile tail. Phage STWB21 is relatively stable under a wide range of pH (4-11) and temperatures (4°C-50°C) for different serovars. The latent period and burst size of phage STWB21 against were 25 min and 161 plaque-forming units per cell. Since is a foodborne pathogen, the phage STWB21 was applied to treat a 24 h biofilm formed in onion and milk under laboratory conditions. A significant reduction was observed in the bacterial population of biofilm in both cases. Phage STWB21 contained a dsDNA of 112,834 bp in length, and the GC content was 40.37%. Also, genomic analysis confirmed the presence of lytic genes and the absence of any lysogeny or toxin genes. Overall, the present study reveals phage STWB21 has a promising ability to be used as a biocontrol agent of spp. and proposes its application in food industries.
是5岁以下儿童和老年人群中细菌性肠胃炎相关发病和死亡的常见致病因素之一。人类沙门氏菌病主要是由于食用了来自动物的受污染食物所致。该属有多个血清型，最近有报道称其中许多对多种药物具有抗性。因此，分离具有杀菌活性的裂解性噬菌体受到了重视。在本研究中，从湖水样本中分离出一株噬菌体STWB21，发现它是一种新型裂解性噬菌体，对宿主细菌具有良好的潜力。然而，在其广泛的宿主范围中观察到了一些多价性。除了[具体细菌名称1]外，噬菌体STWB21还能够感染[具体细菌名称2]以及其他一些细菌物种，如[具体细菌名称3]和[具体细菌名称4]。新分离的噬菌体STWB21属于[噬菌体家族名称]科，具有二十面体头部和长而灵活的非收缩性尾部。噬菌体STWB21在不同[具体细菌名称]血清型的广泛pH范围（4 - 11）和温度范围（4°C - 50°C）下相对稳定。噬菌体STWB21对[具体细菌名称]的潜伏期和裂解量分别为25分钟和每细胞161个噬菌斑形成单位。由于[具体细菌名称]是一种食源性病原体，在实验室条件下，将噬菌体STWB21应用于处理洋葱和牛奶中形成的24小时生物膜。在这两种情况下，均观察到[具体细菌名称]生物膜中的细菌数量显著减少。噬菌体STWB21含有一条长度为112,834 bp的双链DNA，GC含量为40.37%。此外，基因组分析证实了裂解基因的存在以及任何溶原或毒素基因的缺失。总体而言，本研究表明噬菌体STWB21具有作为[具体细菌名称]属生物防治剂的良好潜力，并提出了其在食品工业中的应用。
