Andrade G B, Ladeira S L, Battlehner C N, Saldiva P H N, Caldini E G
Department of Epidemiology and Public Health, Rural Federal University of Rio de Janeiro, Rio de Janeiro, Brazil.
Vet Res Commun. 2008 Jan;32(1):65-74. doi: 10.1007/s11259-007-9003-0. Epub 2007 May 11.
This work aimed to investigate some aspects related to the pathogenicity of Lechiguana, a bovine fibroproliferative lesion characterized by rapid collagen accumulation. Light and transmission electron microscopy and in situ hybridization studies were performed in order to elucidate the fibrogenic activity of this lesion. The characterization of fibroblastic plasticity in the lesion was done by immunohistochemical study for alpha-smooth-muscle cell actin. The ovoid-shaped cells presented positive reaction for alpha-smooth-muscle cell actin in their cytoplasm and, at the electron-microscopic level demonstrated basal lamina-like material adjacent to the external surface and collagen fibrils that corresponded to a cell population phenotypically similar to the myofibroblast. We also investigated alpha1 collagen type I mRNA at different times of evolution of Lechiguana lesions, using isotopic and non-isotopic in situ hybridization. The results strongly suggest the involvement of a myofibroblast-like cell population that expresses mRNA for type I collagen and is probably associated with the increase of collagen deposition.
这项工作旨在研究与牛纤维增生性病变Lechiguana致病性相关的一些方面，该病变的特征是胶原快速积累。进行了光镜和透射电镜以及原位杂交研究，以阐明该病变的纤维生成活性。通过对α-平滑肌肌动蛋白进行免疫组织化学研究，对病变中的成纤维细胞可塑性进行了表征。卵形细胞在其细胞质中对α-平滑肌肌动蛋白呈阳性反应，并且在电子显微镜水平上显示，外表面附近有基底膜样物质以及与表型类似于肌成纤维细胞的细胞群体相对应的胶原纤维。我们还使用同位素和非同位素原位杂交技术，在Lechiguana病变发展的不同时间研究了I型α1胶原mRNA。结果有力地表明，存在一种表达I型胶原mRNA的肌成纤维细胞样细胞群体，其可能与胶原沉积增加有关。
