He Yan-Jiao, Jia Xin-Shan, Hasui Kazuhisa, Wang En-Hua, He An-Guang
Department of Pathology, China Medical University, Shenyang 110001, China.
Zhonghua Bing Li Xue Za Zhi. 2007 Feb;36(2):94-7.
To study the clinical features, immunophenotypes and the significance of Epstein-Barr virus infection in primary nasal and pharyngeal non-Hodgkin's lymphomas in Shenyang.
One hundred and fifty eight cases of primary nasal and pharyngeal non-Hodgkin's lymphomas were included in this study. The samples were stained with haematoxylin and eosin for histological examination. Immunohistochemistry studies were performed using monoclonal antibodies, including CD3 for T-lymphocytes, CD20 for B-lymphocytes, and CD56 and CD57 for NK cells. All cases were reclassified according to the new WHO classification of lymphomas (2001). In situ hybridization detection of EBV-encoded small nuclear RNA (EBER-1) was performed in 99 cases.
Overall, 101 (63.9%) of the 158 NHL were extranodal NK/T cell lymphomas (nasal type), 23 (14.6%) were nonspecific peripheral T cell lymphomas and the remaining 34 cases (21.5%) were B cell lymphomas. The primary sites of involvement were the nasal cavity (53.2%, 84/158), the tonsil (24.7%, 39/158) and the pharynx (22.1%, 35/158). Among 99 cases studied by EBER-1 in situ hybridization, a positive detection was seen in 70/71 cases (98.6%) of extranodal NK/T cell lymphoma (nasal type), 8/12 cases (66.7%) of T cell lymphoma, and 7/16 cases (43.8%) of B cell lymphoma.
Among primary nasal and pharyngeal NK lymphomas, extranodal NK/T cell lymphoma (nasal type) is the most common type and is strongly associated with EBV infection. The pathological diagnosis of nasal and pharyngeal lymphomas should take considerations of the anatomic sites and immunophenotypical features.
研究沈阳地区原发性鼻及咽非霍奇金淋巴瘤的临床特征、免疫表型及爱泼斯坦-巴尔病毒感染的意义。
本研究纳入158例原发性鼻及咽非霍奇金淋巴瘤病例。样本进行苏木精-伊红染色以进行组织学检查。使用单克隆抗体进行免疫组织化学研究，包括用于T淋巴细胞的CD3、用于B淋巴细胞的CD20以及用于NK细胞的CD56和CD57。所有病例均根据世界卫生组织淋巴瘤新分类（2001年）重新分类。对99例病例进行了爱泼斯坦-巴尔病毒编码小核RNA（EBER-1）的原位杂交检测。
总体而言，158例非霍奇金淋巴瘤中，101例（63.9%）为结外NK/T细胞淋巴瘤（鼻型），23例（14.6%）为非特异性外周T细胞淋巴瘤，其余34例（21.5%）为B细胞淋巴瘤。主要受累部位为鼻腔（53.2%，84/158）、扁桃体（24.7%，39/158）和咽部（22.1%，35/158）。在99例通过EBER-1原位杂交研究的病例中，结外NK/T细胞淋巴瘤（鼻型）71例中有70例（98.6%）检测呈阳性，T细胞淋巴瘤12例中有8例（66.7%）阳性，B细胞淋巴瘤16例中有7例（43.8%）阳性。
在原发性鼻及咽NK淋巴瘤中，结外NK/T细胞淋巴瘤（鼻型）是最常见的类型，且与爱泼斯坦-巴尔病毒感染密切相关。鼻及咽淋巴瘤的病理诊断应考虑解剖部位和免疫表型特征。
