人血清白蛋白支持的用于大肠杆菌识别的糖脂模式。

Glycolipid patterns supported by human serum albumin for E. coli recognition.

作者信息

Zhang Xiaoming, He Qiang, Yan Xuehai, Boullanger Paul, Li Junbai

机构信息

Beijing National Lab for Molecular Sciences, Key Lab of Colloid and Interface Science, The Center for Molecular Sciences, Institute of Chemistry, Chinese Academy of Sciences, Zhong Guan Cun, Bei Yi Jie 2, Beijing, China.

出版信息

Biochem Biophys Res Commun. 2007 Jun 29;358(2):424-8. doi: 10.1016/j.bbrc.2007.04.154. Epub 2007 May 2.

DOI:10.1016/j.bbrc.2007.04.154

PMID:17493583

Abstract

We demonstrated that human serum albumin (HSA) patterns constructed in a solid substrate by using micro-contact printing (muCP) technique supported the deposition of phospholipid bilayer containing glycolipid, 10-tetradecyloxymethy-3,6,9,12-tetraoxahexacosyl 2-acetamido-2-deoxy-beta-D-glucopyranoside (PB1124). It is observed by confocal laser scanning microscopy (CLSM) that the obtained glycolipid patterns are well-defined, stable and can be used to recognize and immobilize Escherichia coli (E. coli). This strategy is promising to perform bacterial detection through solid surface recognition in a way of biosensors.

摘要

我们证明，通过微接触印刷（μCP）技术在固体基质中构建的人血清白蛋白（HSA）图案支持含有糖脂10-十四烷氧基甲基-3,6,9,12-四氧杂二十六烷基2-乙酰氨基-2-脱氧-β-D-吡喃葡萄糖苷（PB1124）的磷脂双层的沉积。通过共聚焦激光扫描显微镜（CLSM）观察到，所获得的糖脂图案清晰、稳定，可用于识别和固定大肠杆菌（E. coli）。这种策略有望通过生物传感器的方式通过固体表面识别来进行细菌检测。