Montagnana Martina, Volpe Alessandro, Lippi Giuseppe, Caramaschi Paola, Salvagno Gian Luca, Biasi Domenico, Bambara Lisa Maria, Guidi Gian Cesare
Sezione di Chimica e Microscopia Clinica, Dipartimento di Scienze Morfologico-Biomediche, Università degli Studi di Verona, Ospedale Policlinico G B Rossi, Piazzale Scuro, 10, Verona, Italy.
Clin Biochem. 2007 Aug;40(12):837-42. doi: 10.1016/j.clinbiochem.2007.03.023. Epub 2007 Apr 19.
Systemic sclerosis (SSc) is a connective tissue disease characterized by tissue fibrosis that reflects an imbalance between collagen production and degradation. Matrix metalloproteinases (MMPs) are a family of endopeptidases involved in the remodelling of extracellular matrix (ECM). This activity is controlled by tissue inhibitors of MMP (TIMPs). Aim of this study was the evaluation of MMP-9/TIMP-1 and MMP-2/TIMP-2 systems in patients with SSc.
SearchLight Human MMP Array 1 was used to measure MMPs and TIMPs in 32 SSc patients and 32 matched healthy controls.
SSc patients showed higher values of both MMP-9 and TIMP-1 in comparison with controls. The patients with anticentromere antibodies (ACA) positivity showed higher values of MMPs and TIMPs in comparison with either controls or the patients with anti-Scl70-positive antibodies.
Results of this investigation suggest that SSc patients with ACA positivity, after a primary fibrogenetic noxa, react with a more abundant release of MMP/TIMP, whereas patients with anti-Scl70 antibody show a normal response.
系统性硬化症（SSc）是一种以组织纤维化为特征的结缔组织疾病，这反映了胶原蛋白生成与降解之间的失衡。基质金属蛋白酶（MMPs）是一类参与细胞外基质（ECM）重塑的内肽酶。这种活性受MMP组织抑制剂（TIMPs）控制。本研究的目的是评估SSc患者的MMP-9/TIMP-1和MMP-2/TIMP-2系统。
使用SearchLight人类MMP阵列1检测32例SSc患者和32例匹配的健康对照者的MMPs和TIMPs。
与对照组相比，SSc患者的MMP-9和TIMP-1值均较高。与对照组或抗Scl70抗体阳性患者相比，抗着丝点抗体（ACA）阳性患者的MMPs和TIMPs值更高。
本研究结果表明，ACA阳性的SSc患者在原发性致纤维化损伤后，MMP/TIMP释放更为丰富，而抗Scl70抗体患者表现出正常反应。
