Zeng Shao Ju, Song Kuijin, Xu Na, Zhang Xin Wen, Zuo Ming Xue
Key Laboratory for Cell Proliferation And Regulation Biology, Ministry of Education, Beijing Normal University, Beijing 100875, China.
Neurosci Res. 2007 Jun;58(2):207-14. doi: 10.1016/j.neures.2007.02.001. Epub 2007 Feb 8.
Cellular proliferation within the ventricular zone (VZ) may contribute to sex differences through the net addition of neurons in song control nuclei. To address this issue, we administered [(3)H]thymidine to Bengalese finches of both sexes, and estradiol benzoate (EB) to females 15 days post hatching. The birds were killed 2h later to examine thymidine labeled cells within the VZ at three brain levels, HVC, anterior commissure and Area X. Our results indicated that: (1) cell proliferation in the VZ was significantly higher in the three studied brain levels in males and EB implant females relative to intact or empty implant females, respectively; (2) proliferation in the dorsal half of the VZ, in proximity to HVC, was notably higher than that in the ventral half of the VZ; (3) proliferation in the ventral VZ (VVZ), which is relatively close to Area X was higher relative to other subregions of VZ (dorsal and intermediate). Our study suggests that sex differences in cell proliferation in the VZ may contribute to the net growth of HVC and Area X in males, and estradiol may play an important role in sexual difference in cellular proliferation within the VZ.
脑室区（VZ）内的细胞增殖可能通过在鸣叫控制核中神经元的净增加来导致性别差异。为了解决这个问题，我们给雌雄孟加拉雀都注射了[³H]胸腺嘧啶核苷，并在孵化后15天给雌性注射苯甲酸雌二醇（EB）。2小时后处死这些鸟，以检查三个脑区水平（HVC、前连合和X区）的VZ内胸腺嘧啶核苷标记的细胞。我们的结果表明：（1）相对于完整或假植入雌性，雄性和EB植入雌性在三个研究脑区水平的VZ中的细胞增殖分别显著更高；（2）靠近HVC的VZ背侧半部分的增殖明显高于VZ腹侧半部分；（3）相对于VZ的其他亚区（背侧和中间），相对靠近X区的腹侧VZ（VVZ）的增殖更高。我们的研究表明，VZ中细胞增殖的性别差异可能有助于雄性HVC和X区的净生长，并且雌二醇可能在VZ内细胞增殖的性别差异中起重要作用。
