用DNA修饰的预处理玻碳电极电化学测定痕量盐酸异丙嗪

Electrochemical determination of trace promethazine hydrochloride by a pretreated glassy carbon electrode modified with DNA.

作者信息

Yang Zhou-Sheng, Zhao Jun, Zhang Da-Peng, Liu Yun-Chun

机构信息

College of Chemistry and Materials Science, Anhui Key Laboratory of Functional Molecular Solids, Anhui Normal University, P. R. China.

出版信息

Anal Sci. 2007 May;23(5):569-72. doi: 10.2116/analsci.23.569.

DOI:10.2116/analsci.23.569

PMID:17495403

Abstract

A highly sensitive electrochemical biosensor for the detection of trace amounts of promethazine has been designed. Double stranded (ds)DNA molecules are immobilized onto a pretreated glassy carbon electrode (GCE(ox)) surface. The voltammetric behaviors of promethazine on DNA-modified electrode were explored by means of cyclic voltammetry (CV) and differential pulse voltammetry (DPV). The promethazine gave rise to a pair of well-defined peaks, which appeared at E(pc) = 52 mV and E(pa) = 96 mV (vs. Ag/AgCl) in 0.10 M acetate buffer (pH 5.0). The peak current was linearly enhanced with increasing the concentration of promethazine. The calibration was linear for promethazine over the range of 4.7 x 10(-10) to 9.3 x 10(-9) M with a correlation coefficient of 0.999. The limit of detection (LODs) was 3.0 x 10(-10) M (S/N = 3). The modified electrode was applied to determine promethazine in human blood samples with satisfactory results.

摘要

设计了一种用于检测痕量异丙嗪的高灵敏度电化学生物传感器。双链（ds）DNA分子固定在预处理的玻碳电极（GCE(ox)）表面。采用循环伏安法（CV）和差分脉冲伏安法（DPV）研究了异丙嗪在DNA修饰电极上的伏安行为。异丙嗪产生了一对清晰的峰，在0.10 M醋酸盐缓冲液（pH 5.0）中，其出现在E(pc) = 52 mV和E(pa) = 96 mV（相对于Ag/AgCl）处。随着异丙嗪浓度的增加，峰电流呈线性增强。异丙嗪在4.7×10(-10)至9.3×10(-9) M范围内校准曲线呈线性，相关系数为0.999。检测限（LODs）为3.0×10(-10) M（S/N = 3）。该修饰电极用于测定人体血液样本中的异丙嗪，结果令人满意。