Tabe Misa, Fujimoto Tsuyoshi, Nakahara Ryosuke, Yamaguchi Takako, Fujita Yoshikazu
Osaka University of Pharmaceutical Sciences, Osaka, Japan.
Anal Sci. 2007 May;23(5):601-4. doi: 10.2116/analsci.23.601.
A simple and rapid spectrophotometric method for the determination of oxalate ion was established by the fading of a colored complex between N,N'-diethyl-N,N'-[[4,4'-dihydroxy-1,1'-binaphthalene]-3,3'-diyl]bisbenzamide and copper(II). Beer's law was obeyed in the concentration range of 0.1 - 2.0 microg cm(-3) for oxalate ion, with an effective molar absorptivity at 533 nm and the relative standard deviation being 8.0 x 10(3) dm(3) mol(-1) cm(-1) and 1.0% (n = 5), respectively. This proposed method has excellent reproducibility, and was applied to recovery tests of oxalate ion in tap water and human urine; the results were satisfactory. This is suggested that the method is based on the reaction of copper(II) to copper(I) with oxalate ion.
建立了一种简单快速的分光光度法，用于测定草酸根离子，该方法基于N,N'-二乙基-N,N'-[[4,4'-二羟基-1,1'-联萘]-3,3'-二基]双苯甲酰胺与铜（II）形成的有色配合物的褪色反应。草酸根离子在0.1 - 2.0 μg cm(-3)浓度范围内符合比尔定律，在533 nm处的有效摩尔吸光系数为8.0 x 10(3) dm(3) mol(-1) cm(-1)，相对标准偏差为1.0%（n = 5）。该方法具有良好的重现性，并应用于自来水和人尿中草酸根离子的回收率测试；结果令人满意。这表明该方法基于草酸根离子将铜（II）还原为铜（I）的反应。
