Gouk Sok-Siam, Lim Tit-Meng, Teoh Swee-Hin, Sun Wendell Q
Department of Biological Sciences, National University of Singapore, Singapore.
J Biomed Mater Res B Appl Biomater. 2008 Jan;84(1):205-17. doi: 10.1002/jbm.b.30862.
AlloDerm, a processed acellular human tissue matrix, is used in a number of surgical applications for tissue repair and regeneration. In the present work, AlloDerm serves as a model system for studying gamma radiation-induced changes in tissue structure and stability as well as the effect of such changes on the cell-matrix interactions, including cell repopulation and matrix remodeling. AlloDerm tissue matrix was treated with 2-30 kGy gamma irradiation at room temperature. Gamma irradiation reduced the swelling of tissue matrix upon rehydration and caused significant structural modifications, including collagen condensation and hole formation in collagen fibres. The tensile strength of AlloDerm increased at low gamma dose but decreased with increasing gamma dosage. The elasticity of irradiated AlloDerm was reduced significantly. Calorimetric study showed that gamma irradiation destabilized the tissue matrix, resulting in greater susceptibility to proteolytic enzyme degradation. Although gamma irradiation did not affect in vitro proliferation of fibroblast cells, it promoted tissue degradation upon cell repopulation and influenced synthesis and deposition of new collagen.
同种异体脱细胞真皮基质（AlloDerm）是一种经过处理的无细胞人类组织基质，用于多种组织修复和再生的外科手术应用中。在本研究中，AlloDerm作为一个模型系统，用于研究γ射线辐射引起的组织结构和稳定性变化，以及这些变化对细胞-基质相互作用的影响，包括细胞再填充和基质重塑。在室温下，将AlloDerm组织基质用2-30千戈瑞的γ射线进行辐照。γ射线辐照降低了组织基质再水化后的肿胀，并引起了显著的结构改变，包括胶原纤维的凝聚和孔洞形成。AlloDerm的拉伸强度在低γ剂量下增加，但随着γ剂量的增加而降低。辐照后的AlloDerm弹性显著降低。量热研究表明，γ射线辐照使组织基质不稳定，导致其对蛋白水解酶降解的敏感性增加。虽然γ射线辐照不影响成纤维细胞的体外增殖，但在细胞再填充时它促进了组织降解，并影响了新胶原的合成和沉积。
