Wang Bin, Qinglai Tang, Yang Qian, Li Mengmeng, Zeng Shiying, Yang Xinming, Xiao Zian, Tong Xinying, Lei Lanjie, Li Shisheng
Department of Otorhinolaryngology Head and Neck Surgery, The Second Xiangya Hospital, Central South University, Changsha 410011, China.
Department of Hemodialysis, The Second Xiangya Hospital, Central South University, Changsha 410011, Hunan, China.
Mater Today Bio. 2022 Dec 28;18:100530. doi: 10.1016/j.mtbio.2022.100530. eCollection 2023 Feb.
In view of their low immunogenicity, biomimetic internal environment, tissue- and organ-like physicochemical properties, and functionalization potential, decellularized extracellular matrix (dECM) materials attract considerable attention and are widely used in tissue engineering. This review describes the composition of extracellular matrices and their role in stem-cell differentiation, discusses the advantages and disadvantages of existing decellularization techniques, and presents methods for the functionalization and characterization of decellularized scaffolds. In addition, we discuss progress in the use of dECMs for cartilage, skin, nerve, and muscle repair and the transplantation or regeneration of different whole organs (e.g., kidneys, liver, uterus, lungs, and heart), summarize the shortcomings of using dECMs for tissue and organ repair after refunctionalization, and examine the corresponding future prospects. Thus, the present review helps to further systematize the application of functionalized dECMs in tissue/organ transplantation and keep researchers up to date on recent progress in dECM usage.
鉴于其低免疫原性、仿生内部环境、组织和器官样的物理化学性质以及功能化潜力，脱细胞细胞外基质（dECM）材料备受关注并广泛应用于组织工程。本综述描述了细胞外基质的组成及其在干细胞分化中的作用，讨论了现有脱细胞技术的优缺点，并介绍了脱细胞支架功能化和表征的方法。此外，我们讨论了dECM在软骨、皮肤、神经和肌肉修复以及不同全器官（如肾脏、肝脏、子宫、肺和心脏）移植或再生方面的应用进展，总结了再功能化后使用dECM进行组织和器官修复的缺点，并审视了相应的未来前景。因此，本综述有助于进一步系统化功能化dECM在组织/器官移植中的应用，并使研究人员了解dECM使用的最新进展。
