Pan Guang-bing, Song Fu-yong, Zhao Xiu-lan, Yu Li-hua, Zhou Gui-zhen, Xie Ke-qin
Institute of Toxicology, School of Public Health, Shandong University, Jinan 250012, China.
Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2007 Mar;25(3):148-51.
To investigate whether the alterations of microtubule and microfilament expression are responsible for the neurotoxicity of carbon disulfide.
Wistar rats were administered with carbon disulfide by gavage at a dosage of 300 or 500 mg/kg for continuous 12 weeks (five times per week). Spinal cords of carbon disulfide-intoxicated rats and their age-matched controls were Triton-extracted and ultracentrifuged to yield a pellet and a corresponding supernatant fraction. Then, the contents of alpha-tubulin, beta-tubulin, and beta-actin in both fractions were determined by immunoblotting. In the meantime, their mRNA levels in spinal cords were quantified using reverse transcriptase-polymerase chain reaction (RT-PCR).
In the supernatant fraction, the contents of beta-tubulin and beta-actin in both treated groups increased significantly (P < 0.01) the content of beta-tubulin increased by 141% and 158% respectively, and the content of beta-actin increased by 19% and 32% respectively. In the pellet fraction, the content of beta-tubulin in both groups increased by 107%(P < 0.01) and 118%(P < 0.01) respectively, and the others keep unaffected. In the meantime, the levels of of mRNA expression of beta-tubulin and beta-actin gene were elevated consistently in CS(2)-treated groups (P < 0.01) the levels of mRNA expression of beta-tubulin increased by 207% and 212% respectively, and the levels of mRNA expression of beta-actin increased by 94% and 91% respectively.
Carbon disulfide intoxication results in alternations of microtubule and microfilament expression, and the alternations might be related to its neurotoxicity.
探讨微管和微丝表达的改变是否与二硫化碳的神经毒性有关。
将Wistar大鼠按300或500mg/kg的剂量经口灌胃给予二硫化碳，连续12周（每周5次）。对二硫化碳中毒大鼠及其年龄匹配的对照大鼠的脊髓进行Triton抽提和超速离心，得到沉淀和相应的上清部分。然后，通过免疫印迹法测定两部分中α-微管蛋白、β-微管蛋白和β-肌动蛋白的含量。同时，使用逆转录聚合酶链反应（RT-PCR）对脊髓中它们的mRNA水平进行定量。
在上清部分，两个处理组中β-微管蛋白和β-肌动蛋白的含量均显著增加（P<0.01），β-微管蛋白的含量分别增加了141%和158%，β-肌动蛋白的含量分别增加了19%和32%。在沉淀部分，两组中β-微管蛋白的含量分别增加了107%（P<0.01）和118%（P<0.01），其他成分未受影响。同时，在二硫化碳处理组中，β-微管蛋白和β-肌动蛋白基因的mRNA表达水平持续升高（P<0.01），β-微管蛋白的mRNA表达水平分别增加了207%和212%，β-肌动蛋白的mRNA表达水平分别增加了94%和91%。
二硫化碳中毒导致微管和微丝表达的改变，这些改变可能与其神经毒性有关。
