Karajan Bella P, Vishnyakov Andrey E, Tavrovskaya Marina V, Vasyanin Sergey I
Institute of Cytology, Russian Academy of Sciences, Tikhorezky Avenue 4, 194064 St. Petersburg, Russian Federation.
Eur J Protistol. 2007 Jun;43(2):141-6. doi: 10.1016/j.ejop.2007.01.001. Epub 2007 May 10.
The ciliate Climacostomum virens normally contains algae as symbionts in its cytoplasm and retains them over many generations. An aposymbiotic strain of C. virens which cannot re-establish a new symbiotic association by ingestion of algae derived from green Climacostomum was recently isolated in our laboratory. Results of infection experiments showed that all newly ingested, potentially symbiotic algae were digested in food vacuoles. To clarify whether these ciliates have completely lost their ability to sustain symbiosis with algae or whether this ability can eventually be re-established, infection experiments were performed using a microinjection technique. We have achieved successful infection of algae-free Climacostomum using this method. The endosymbiont population was established in ciliates from as few as 3-5 injected algae, which have retained an intact perialgal vacuole membrane around them. To our knowledge, this is the first evidence of successful infection of aposymbiotic ciliates with algae by microinjection.
纤毛虫绿口列虫（Climacostomum virens）的细胞质中通常含有作为共生体的藻类，并能在许多代中保留它们。最近，我们实验室分离出了一种绿口列虫的无共生体菌株，该菌株无法通过摄取源自绿色绿口列虫的藻类来重新建立新的共生关系。感染实验结果表明，所有新摄取的、潜在的共生藻类都在食物泡中被消化。为了阐明这些纤毛虫是完全丧失了与藻类维持共生关系的能力，还是这种能力最终可以重新建立，我们使用显微注射技术进行了感染实验。通过这种方法，我们成功地使无藻绿口列虫受到了感染。从仅3 - 5个注射的藻类开始，内共生体群体就在纤毛虫中建立起来，这些藻类周围保留着完整的围藻泡膜。据我们所知，这是通过显微注射使无共生体纤毛虫成功感染藻类的首个证据。
