Lévêque Céline, Marsaud Véronique, Renoir Jack-Michel, Sola Brigitte
Laboratoire de Biologie Moléculaire et Cellulaire de la Signalisation, Université de Caen Basse-Normandie, France.
Exp Cell Res. 2007 Jul 15;313(12):2719-29. doi: 10.1016/j.yexcr.2007.04.018. Epub 2007 Apr 24.
Cyclin D1 is an important regulator of the early phase of the cell cycle and the transcriptional machinery. It is often deregulated in human tumors of various origins and is considered to be an oncogene. The CCND1 gene encoding cyclin D1 generates two mRNAs by alternative splicing, leading to the production of two alternative proteins: a long form a (36 kDa) and a short form b (30-31 kDa) from which the C-terminal moiety required for protein stability and sub-cellular localization has been deleted. Both forms of RNA and protein have been detected in B-cell hemopathies, but their respective roles are unclear. We investigated the function of cyclin D1b in cell cycle regulation, by generating B-cell lines displaying conditional expression of isoform b. Comparisons of these cell lines (BD1b series) with previously obtained cell lines expressing cyclin D1a demonstrated that cyclin D1b had no cell cycle regulatory properties.
细胞周期蛋白D1是细胞周期早期阶段和转录机制的重要调节因子。它在各种起源的人类肿瘤中经常失调，被认为是一种癌基因。编码细胞周期蛋白D1的CCND1基因通过可变剪接产生两种mRNA，导致产生两种可变蛋白：长形式a（36 kDa）和短形式b（30 - 31 kDa），其中蛋白质稳定性和亚细胞定位所需的C末端部分已被删除。在B细胞血液病中已检测到RNA和蛋白质的两种形式，但它们各自的作用尚不清楚。我们通过生成显示异构体b条件性表达的B细胞系，研究了细胞周期蛋白D1b在细胞周期调控中的功能。将这些细胞系（BD1b系列）与先前获得的表达细胞周期蛋白D1a的细胞系进行比较，结果表明细胞周期蛋白D1b没有细胞周期调节特性。
