Hajdú P, Ikemoto T, Akazome Y, Park M K, Oka Y
Laboratory of Biological Signalling, Department of Biological Sciences, Graduate School of Science, The University of Tokyo, Tokyo, Japan.
J Neuroendocrinol. 2007 Jun;19(6):475-9. doi: 10.1111/j.1365-2826.2007.01553.x.
Gonadotophin-releasing hormone (GnRH) peptide released from the terminal nerve (TN)-GnRH neurones of the dwarf gourami primarily modifies the electrical properties of various neurones, including the TN-GnRH neurones themselves. However, our knowledge on the expression of GnRH receptors (GnRHRs) in the TN-GnRH neurones is still limited. Here, we used the single-cell reverse transcriptase-polymerase chain reaction after whole-cell patch-clamp recording to study the distribution of various GnRHR types expressed in the individual TN-GnRH neurones. We found that TN-GnRH neurones express two of the three types of GnRHRs cloned in the dwarf gourami: GnRHR1-2 and -R2, but not -R1-1. Furthermore, in agreement with our previous findings, all TN-GnRH neurones contained mRNAs of salmon GnRH but not chicken GnRH-II.
来自侏儒斗鱼终末神经（TN）-促性腺激素释放激素（GnRH）神经元释放的GnRH肽主要改变各种神经元的电特性，包括TN-GnRH神经元自身。然而，我们对TN-GnRH神经元中GnRH受体（GnRHRs）表达的了解仍然有限。在此，我们在全细胞膜片钳记录后使用单细胞逆转录聚合酶链反应来研究单个TN-GnRH神经元中表达的各种GnRHR类型的分布。我们发现，TN-GnRH神经元表达侏儒斗鱼中克隆的三种GnRHR类型中的两种：GnRHR1-2和-R2，但不表达-R1-1。此外，与我们之前的发现一致，所有TN-GnRH神经元都含有鲑鱼GnRH的mRNA，但不含有鸡GnRH-II的mRNA。
