Guilgur L G, Strüssmann C A, Somoza G M
Laboratorio de Ictiofisiología y Acuicultura, Instituto de Investigaciones Biotecnológicas-Instituto Tecnológico Chascomús, Chascomús, Buenos Aires, Argentina.
Fish Physiol Biochem. 2009 Mar;35(1):157-66. doi: 10.1007/s10695-008-9215-4. Epub 2008 Apr 17.
The present study examined the differential mRNA expression levels of three forms of GnRH (sGnRH, pjGnRH and cGnRH-II) and two forms of GnRH receptor (pjGnRH-R I and pjGnRH-R II) in the brain, pituitary, and ovaries of pejerrey in relation to the reproductive status. The analysis revealed the presence of significant amounts of mRNA of the three GnRH forms while the ovaries showed only two (sGnRH and pjGnRH). The GnRH receptor II was found ubiquitously in the brain, pituitary, and ovaries while the form I was detected only in the brain. The levels of pjGnRH mRNA in the brain and pjGnRH-R II in the pituitary gland varied in correlation with the ovarian condition. However, brain sGnRH and pjGnRH-R I mRNA levels reached a maximum during early stages of ovarian development. In contrast, the brain levels of cGnRH-II mRNA showed no variation. The present study also shows a good correlation of ovarian sGnRH and pjGnRH-R II mRNA levels with the reproductive condition, suggesting that these molecules are may be involved in the regulation of pejerrey ovarian function.
本研究检测了银汉鱼大脑、垂体和卵巢中三种促性腺激素释放激素（sGnRH、pjGnRH和cGnRH-II）以及两种促性腺激素释放激素受体（pjGnRH-R I和pjGnRH-R II）的差异mRNA表达水平与生殖状态的关系。分析显示三种促性腺激素释放激素形式均存在大量mRNA，而卵巢中仅显示两种（sGnRH和pjGnRH）。促性腺激素释放激素受体II在大脑、垂体和卵巢中普遍存在，而形式I仅在大脑中检测到。大脑中pjGnRH mRNA水平和垂体中pjGnRH-R II水平随卵巢状况而变化。然而，大脑中sGnRH和pjGnRH-R I mRNA水平在卵巢发育早期达到最高。相反，大脑中cGnRH-II mRNA水平无变化。本研究还表明卵巢中sGnRH和pjGnRH-R II mRNA水平与生殖状况具有良好的相关性，表明这些分子可能参与银汉鱼卵巢功能的调节。
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