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绵羊Flt3配体的克隆与测序

Cloning and sequencing of ovine Flt3 ligand.

作者信息

Chan S S M, Lyons N, McConnell I, Blacklaws B A

机构信息

Centre for Veterinary Science, Department of Veterinary Medicine, University of Cambridge, Madingley Road, Cambridge, UK.

出版信息

Int J Immunogenet. 2007 Jun;34(3):167-71. doi: 10.1111/j.1744-313X.2007.00659.x.

DOI:10.1111/j.1744-313X.2007.00659.x
PMID:17504506
Abstract

A cDNA (879 bp) containing the complete open reading frame of ovine Fms-related tyrosine kinase 3 ligand (Flt3-L) was amplified by reverse transcriptase polymerase chain reaction (RT-PCR), cloned and sequenced. The deduced amino acid sequence has 97.6% similarity with bovine Flt3-L isoform 1 and shares the long cytoplasmic domain observed in bovine Flt3-L but not in human Flt3-L.

摘要

通过逆转录聚合酶链反应（RT-PCR）扩增出一个包含绵羊Fms相关酪氨酸激酶3配体（Flt3-L）完整开放阅读框的cDNA（879 bp），并进行克隆和测序。推导的氨基酸序列与牛Flt3-L亚型1具有97.6%的相似性，且具有在牛Flt3-L中观察到但在人Flt3-L中未观察到的长细胞质结构域。

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