Dudley D J, LaMarche S, Mitchell M D
Department of Obstetrics and Gynecology, University of Utah School of Medicine, Salt Lake City 84132.
Am J Obstet Gynecol. 1991 Dec;165(6 Pt 1):1723-6. doi: 10.1016/0002-9378(91)90022-j.
Human umbilical vessels are not innervated, and hence regulation of tone in these vessels must come from locally derived vasoactive substances such as prostacyclin. To evaluate the regulation of prostacyclin production by human umbilical vein endothelial cells, we incubated confluent cultures of these cells with various concentrations of inflammatory mediators (endotoxin, interleukin-1 beta, and tumor necrosis factor), protein kinase C agonists (phorbol 12-myristate 13-acetate, phorbol 12,13-dibutyrate), and calcium ionophores (A-23187 and ionomycin). Human umbilical vein endothelial cells were prepared from term pregnancies, and confluent cultures were incubated with test substances for 16 hours. Prostacyclin was measured as its metabolite 6-keto-prostaglandin F1 alpha by radioimmunoassay. Concentration-related increases in 6-keto-prostaglandin F1 alpha production were observed in response to endotoxin, cytokines, phorbol esters, and calcium ionophores. We conclude that human umbilical vein endothelial cell prostacyclin production is influenced by several intracellular messengers and that human umbilical vein endothelial cells may provide a useful in vitro model for investigating the physiology and pathophysiology of umbilical vessel vascular tone.
人脐血管没有神经支配，因此这些血管张力的调节必定来自局部产生的血管活性物质，如前列环素。为了评估人脐静脉内皮细胞对前列环素产生的调节作用，我们将这些细胞的汇合培养物与不同浓度的炎性介质（内毒素、白细胞介素-1β和肿瘤坏死因子）、蛋白激酶C激动剂（佛波醇12-肉豆蔻酸酯13-乙酸酯、佛波醇12,13-二丁酸酯）和钙离子载体（A-23187和离子霉素）一起孵育。人脐静脉内皮细胞取自足月妊娠，汇合培养物与测试物质孵育16小时。通过放射免疫测定法将前列环素作为其代谢产物6-酮-前列腺素F1α进行测量。观察到响应内毒素、细胞因子、佛波酯和钙离子载体，6-酮-前列腺素F1α产生呈浓度相关增加。我们得出结论，人脐静脉内皮细胞前列环素的产生受几种细胞内信使的影响，并且人脐静脉内皮细胞可能为研究脐血管血管张力的生理学和病理生理学提供一个有用的体外模型。
