生物素化碱性成纤维细胞生长因子具有生物活性。

Biotinylated basic fibroblast growth factor is biologically active.

作者信息

Pieri I, Barritault D

机构信息

Laboratoire de Biotechnologie des Cellules Eucaryotes, Université Paris XII, Créteil, France.

出版信息

Anal Biochem. 1991 Jun;195(2):214-9. doi: 10.1016/0003-2697(91)90319-o.

DOI:10.1016/0003-2697(91)90319-o

PMID:1750669

Abstract

Basic fibroblast growth factor (bFGF) was modified by biotinylation via amino group substitution, using biotin-N-hydroxysuccinimide ester at molar reaction ratios of 20, 200, and 2000 per bFGF molecule (respectively named bio-bFGF.20, bio-bFGF.200, and bio-bFGF.2000). The biotinylated bFGF derivatives, bio-bFGF.20 and bio-bFGF.200, conserved the same affinity for heparin as native bFGF, in contrast to bio-FGF.2000 which lost this property. Bio-bFGF.20 and bio-bFGF.200 were as effective as native bFGF in their capacity to compete with 125I-bFGF for binding to bFGF receptor on bovine brain membranes. The biological activity of these bFGF derivatives was tested on CCL39 cells; bio-bFGF.20 and bio-bFGF.200 were as able as native bFGF to promote growth of CCL39.

摘要

通过氨基取代进行生物素化修饰碱性成纤维细胞生长因子（bFGF），使用生物素 - N - 羟基琥珀酰亚胺酯，其与每个bFGF分子的摩尔反应比分别为20、200和2000（分别命名为bio - bFGF.20、bio - bFGF.200和bio - bFGF.2000）。生物素化的bFGF衍生物bio - bFGF.20和bio - bFGF.200对肝素的亲和力与天然bFGF相同，而bio - FGF.2000则失去了这一特性。Bio - bFGF.20和bio - bFGF.200在与125I - bFGF竞争结合牛脑膜上的bFGF受体的能力方面与天然bFGF一样有效。这些bFGF衍生物的生物活性在CCL39细胞上进行了测试；bio - bFGF.20和bio - bFGF.200促进CCL39生长的能力与天然bFGF相同。