Ohi Satoshi, Takahashi Naoto, Ninomiya Kouzou, Nakajima Masako, Hashimoto Hisashi, Tachibana Toshiaki, Yanaga Katsuhiko, Ishikawa Hiroshi
Department of Anatomy II, Jikei Univesity School of Medicine, Japan.
Hum Cell. 2007 Feb;20(1):15-22. doi: 10.1111/j.1749-0774.2007.00024.x.
We successfully established a spontaneously cisplatin-resistant tumor cell line (designated as IGSK-1) derived from original gastric carcinoma. The patient was a 75-year-old Japanese woman. The histopathological diagnosis was gastric poorly differentiated adenocarcinoma accompanied with metastatic foci in lymph nodes, pT3, N2 M0, stage IIIB. The IGSK-1 cells grew as adhesive and monolayered cultures on the bottom of dishes. The susceptibility of the IGSK-1 cells to anti-cancer drugs was examined using oxygen electrode apparatus (Daikin, Tsukuba, JPN), and the results suggested TXL was effective, and CDDP, CPT-11 and 5-FU were not effective. Gastrin and somatostatin secretions were confirmed by immunohistochemical staining and also radioimmunoassay. Immunohistochemistry and radioimmunoassay for serotonin suggested the IGSK-1 cells might incorporate serotonin from the growth media. Spontaneously cisplatin-resistant gastric carcinoma cell line secreted gastrin and somatostatin is very important material for chemotherapy.
我们成功建立了一种源自原发性胃癌的顺铂自发耐药肿瘤细胞系（命名为IGSK-1）。该患者为一名75岁的日本女性。组织病理学诊断为胃低分化腺癌伴淋巴结转移灶，pT3，N2 M0，IIIB期。IGSK-1细胞在培养皿底部以贴壁单层培养的方式生长。使用氧电极装置（大金，筑波，日本）检测了IGSK-1细胞对抗癌药物的敏感性，结果表明TXL有效，而顺铂、CPT-11和5-FU无效。通过免疫组织化学染色和放射免疫测定证实了胃泌素和生长抑素的分泌。血清素的免疫组织化学和放射免疫测定表明IGSK-1细胞可能从生长培养基中摄取血清素。顺铂自发耐药的胃癌细胞系分泌胃泌素和生长抑素是化疗的非常重要的物质。
