Terano A, Nakada R, Mutoh H, Hiraishi H, Ota S, Kimura K, Aoyama H, Sugimoto T, Shiga J, Itoh Y
Second Department of Medicine, School of Medicine, University of Tokyo, Japan.
Hum Cell. 1989 Sep;2(3):307-9.
Despite the importance of in vitro study of gastric cancer, the established cell lines derived from human gastric carcinoma are very few. We have recently established a new cell line derived from human gastric cancer which has the ability to produce several tumor markers. This cell line has been designated JR-1. The cancer cells were obtained from the cerebrospinal fluid of a 37 year-old female patient who had metastatic brain tumor of the poorly differentiated gastric adenocarcinoma. The cells were inoculated into the tissue culture flask containing Ham's F-12 medium supplemented with 10% fetal bovine serum, antibiotics. Within 24 hours, the cells attached to the surface of the flask and started to grow. The first subcultures were performed at 1 week, and subsequent subcultures have been done once a week. This cell line has been maintained for more than 15 months through 60 passages with a stable growth. Chromosome analysis of the cells was performed. The doubling time of the 20th passage was 72 hours. Under phase contrast microscopy, monolayered pavement-like cell arrangement was observed. PAS staining showed intracellular mucin granules. Transmission and scanning electron microscopy revealed spindle-shaped cells with numerous microvilli and fine projections as well as intracellular granules, indicating mucin. Tumor markers produced by this cell line were CEA, CA19-9, TPA and Procollagen III.
尽管胃癌的体外研究很重要，但源自人类胃癌的已建立细胞系却非常少。我们最近建立了一种源自人类胃癌的新细胞系，它能够产生多种肿瘤标志物。该细胞系被命名为JR - 1。癌细胞取自一名37岁女性患者的脑脊液，该患者患有低分化胃腺癌的脑转移瘤。将细胞接种到含有补充了10%胎牛血清和抗生素的Ham's F - 12培养基的组织培养瓶中。在24小时内，细胞附着在培养瓶表面并开始生长。第一次传代在1周时进行，随后每周传代一次。该细胞系通过60次传代已稳定生长超过15个月。对细胞进行了染色体分析。第20代的倍增时间为72小时。在相差显微镜下，观察到单层铺路石样细胞排列。PAS染色显示细胞内有粘蛋白颗粒。透射电子显微镜和扫描电子显微镜显示纺锤形细胞，有许多微绒毛和细小突起以及细胞内颗粒，表明有粘蛋白。该细胞系产生的肿瘤标志物有癌胚抗原（CEA）、糖类抗原19 - 9（CA19 - 9）、组织多肽抗原（TPA）和Ⅲ型前胶原。
