Rad50参与有丝分裂细胞中由甲基磺酸甲酯诱导的同源染色体间的重组。

Rad50 is involved in MMS-induced recombination between homologous chromosomes in mitotic cells.

作者信息

Tomizawa Yuji, Ui Ayako, Onoda Fumitoshi, Ogiwara Hideaki, Tada Shusuke, Enomoto Takemi, Seki Masayuki

机构信息

Molecular Cell Biology Laboratory, Graduate School of Pharmaceutical Sciences, Tohoku University, Miyagi, Japan.

出版信息

Genes Genet Syst. 2007 Apr;82(2):157-60. doi: 10.1266/ggs.82.157.

DOI:10.1266/ggs.82.157

PMID:17507781

Abstract

The structural maintenance of chromosomes (SMC) family proteins (Smc1-Smc6) typically consist of two coiled-coil domains, an amino-terminal head domain, and a carboxyl-terminal tail domain. Rad50, a component of the Mre11/Rad50/Xrs2 (MRX) complex, has a similar domain structure to the SMC proteins. In Saccharomyces cerevisiae, the MRX complex appears to be essential for recombination between homologous chromosomes in meiotic cells, but not in cells undergoing vegetative growth. Here we provide for the first time evidence that Rad50, like Smc6, is required for the induction of recombination between homologous chromosomes in cells in the vegetative growth state upon exposure to methyl methanesulfonate. However, UV-induced recombination between homologous chromosomes is intact in both rad50 and smc6-56 mutant cells.

摘要

染色体结构维持（SMC）家族蛋白（Smc1 - Smc6）通常由两个卷曲螺旋结构域、一个氨基末端头部结构域和一个羧基末端尾部结构域组成。Rad50是Mre11/Rad50/Xrs2（MRX）复合物的一个组分，其结构域结构与SMC蛋白相似。在酿酒酵母中，MRX复合物对于减数分裂细胞中同源染色体之间的重组似乎至关重要，但对于营养生长的细胞则不然。在此，我们首次提供证据表明，与Smc6一样，Rad50是营养生长状态的细胞在暴露于甲磺酸甲酯后诱导同源染色体之间重组所必需的。然而，在rad50和smc6 - 56突变细胞中，紫外线诱导的同源染色体之间的重组是完整的。