Arduini Fabiana, Amine Aziz, Moscone Danila, Ricci Francesco, Palleschi Giuseppe
Dipartimento di Scienze e Tecnologie Chimiche, Università di Roma Tor Vergata, Via della Ricerca Scientifica, Rome, Italy.
Anal Bioanal Chem. 2007 Jul;388(5-6):1049-57. doi: 10.1007/s00216-007-1330-z. Epub 2007 May 17.
The nerve agents are chemical warfare agents known to be used during terrorist attacks. An inexpensive and portable system to be used by first responders and military personnel is of interest owing to the continuing threat of possible terrorist attacks. Amperometric biosensors based on cholinesterase inhibition show such potentialities. In this work butyrylcholinesterase was immobilized onto screen-printed electrodes modified with Prussian blue and the nerve agent detection was performed by measuring the residual activity of enzyme. The optimized biosensor was tested with sarin and VX standard solutions, showing detection limits of 12 and 14 ppb (10% of inhibition), respectively. The enzymatic inhibition was also obtained by exposing the biosensors to sarin in gas phase. Two different concentrations of sarin gas (0.1 and 0.5 mg m(-3)) at different incubation times (from 30 s up to 10 min) were tested. It is possible to detect sarin at a concentration of 0.1 mg m(-3) with 30-s incubation time, with a degree of inhibition of 34%, which match the legal limits (immediate danger to life and health).
神经毒剂是已知在恐怖袭击中使用的化学战剂。由于恐怖袭击的持续威胁，开发一种供急救人员和军事人员使用的廉价便携系统备受关注。基于胆碱酯酶抑制的安培型生物传感器具有这样的潜力。在这项工作中，丁酰胆碱酯酶被固定在普鲁士蓝修饰的丝网印刷电极上，并通过测量酶的残余活性来进行神经毒剂检测。用沙林和VX标准溶液对优化后的生物传感器进行了测试，其检测限分别为12 ppb和14 ppb（抑制率10%）。通过将生物传感器暴露于气相沙林中也能实现酶抑制。测试了两种不同浓度的沙林气体（0.1和0.5 mg m(-3)）在不同孵育时间（从30秒到10分钟）下的情况。在孵育30秒时能够检测到浓度为0.1 mg m(-3)的沙林，抑制率为34%，这符合法定限值（对生命和健康构成立即危险）。
