Voiushina T L, Terent'eva E Iu, Pozdnev V F, Gaĭda A V, Gololobov M Iu, Liublinskaia L A, Stepanov V M
Bioorg Khim. 1991 Aug;17(8):1066-73.
A method is suggested for synthesis of acylpeptides, containing arginine or lysine p-nitroanilides at the C-terminus, via the acyl transfer reaction catalyzed by the Bacillus subtilis serine proteinase. Acyl-di- and acyltripeptide ethers with L- and D-amino acids were used as the carboxyl component taken in a twofold excess. When the concentration of dimethylformamide increases, the hydrolysis of the initial ether and the reaction product diminishes. Because of the enzyme inactivation by dimethylformamide the latter's optimal concentration is 70-80%.
提出了一种通过枯草芽孢杆菌丝氨酸蛋白酶催化的酰基转移反应合成C末端含有精氨酸或赖氨酸对硝基苯胺的酰基肽的方法。含有L-和D-氨基酸的酰基二肽和酰基三肽醚被用作过量两倍的羧基组分。当二甲基甲酰胺的浓度增加时，初始醚和反应产物的水解减少。由于二甲基甲酰胺会使酶失活，其最佳浓度为70-80%。
