Nakamura Naoya, Ohshima Koichi, Abe Masafumi, Osamura Yoshiyuki
Department of Pathology, Tokai University School of Medicine, Isehara, Japan.
J Clin Exp Hematop. 2007 Apr;47(1):9-13. doi: 10.3960/jslrt.47.9.
We performed single cell polymerase chain reaction (PCR) amplification of the t (14 ; 18) translocation from paraffin embedded sections in a case of follicular lymphoma (FL) with subsequent development of Hodgkin lymphoma (HL). The lymphoma cells of FL were positive for CD20, CD10 and BCL-2, and negative for CD3, CD30 and CD15. Hodgkin and Reed-Sternberg (HRS) cells of HL were positive for CD20, CD30 and CD15, and negative for CD3 and CD10. EBER-1 RNA in situ hybridization failed to stain with both lymphomas. HRS cells manipulated and FL cells micro-shaved from individual neoplastic follicles were subjected to single-cell PCR. The t (14 ; 18) translocation, a chimeric DNA containing portions of the bcl-2 and the immunoglobulin heavy chain (IgH) genes, was amplified from four of 27 isolated HRS cells and two individual FL follicles. All t (14 ; 18) PCRs yielded products of the same size, and an identical nucleotide sequence including the t (14 ; 18) translocation was found in both FL and HRS samples. Thus, the data demonstrate the common clonal origin of FL cells and HRS cells in subsequent HL, and that both FL and HL were derived from germinal center B cells with the t (14 ; 18) translocation.
我们对1例滤泡性淋巴瘤（FL）继发霍奇金淋巴瘤（HL）患者的石蜡包埋切片进行了t（14；18）易位的单细胞聚合酶链反应（PCR）扩增。FL的淋巴瘤细胞CD20、CD10和BCL-2呈阳性，CD3、CD30和CD15呈阴性。HL的霍奇金和里德-斯特恩伯格（HRS）细胞CD20、CD30和CD15呈阳性，CD3和CD10呈阴性。EBER-1 RNA原位杂交对两种淋巴瘤均未染色。对从单个肿瘤性滤泡中处理的HRS细胞和微切的FL细胞进行单细胞PCR。从27个分离的HRS细胞中的4个以及2个单独的FL滤泡中扩增出了t（14；18）易位，即一种包含bcl-2和免疫球蛋白重链（IgH）基因部分的嵌合DNA。所有t（14；18）PCR均产生相同大小的产物，并且在FL和HRS样本中发现了包括t（14；18）易位在内的相同核苷酸序列。因此，数据表明继发HL中的FL细胞和HRS细胞具有共同的克隆起源，并且FL和HL均源自具有t（14；18）易位的生发中心B细胞。
