亲水性维生素E类似物在氧化应激条件下对延伸因子2水平降低的“体外”保护作用。
'In vitro' protective effect of a hydrophilic vitamin E analogue on the decrease in levels of elongation factor 2 in conditions of oxidative stress.
作者信息
Argüelles Sandro, Machado Alberto, Ayala Antonio
机构信息
Departamento de Bioquímica, Bromatología y Toxicología, Facultad de Farmacia, Universidad de Sevilla, Sevilla, España.
出版信息
Gerontology. 2007;53(5):282-8. doi: 10.1159/000102948. Epub 2007 May 16.
BACKGROUND
Protein synthesis is inhibited by oxidative stress. Among the possible causes of this inhibition are the modifications of elongation factor 2 (eEF-2), the protein that catalyzes the translocation of the ribosome through mRNA. eEF-2 is extremely sensitive to oxidative stress caused mainly by lipid peroxidant compounds such as cumene hydroperoxide (CH).
OBJECTIVE
The purpose of this study was to determine whether the antioxidant Trolox prevents the effect of CH on the levels of hepatic eEF-2.
METHODS
The effect was determined in liver homogenates treated with both compounds. Lipid peroxides and carbonyl content were also measured.
RESULTS
The results show that Trolox at certain doses prevents the decrease in the level of eEF-2 caused by CH.
CONCLUSION
Under oxidative stress circumstances, vitamin E can prevent the effect of oxidations on relevant biological processes such as protein synthesis.
背景
蛋白质合成受氧化应激抑制。这种抑制的可能原因包括延伸因子2（eEF - 2）的修饰，eEF - 2是一种催化核糖体通过信使核糖核酸（mRNA）进行易位的蛋白质。eEF - 2对主要由脂质过氧化合物如氢过氧化异丙苯（CH）引起的氧化应激极为敏感。
目的
本研究旨在确定抗氧化剂生育三烯酚（Trolox）是否能预防CH对肝脏eEF - 2水平的影响。
方法
在同时用这两种化合物处理的肝脏匀浆中测定该效应。还测量了脂质过氧化物和羰基含量。
结果
结果表明，一定剂量的Trolox可预防CH引起的eEF - 2水平降低。
结论
在氧化应激情况下，维生素E可预防氧化对诸如蛋白质合成等相关生物学过程的影响。
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