Walker M B, Kimmel C B
Stowers Institute for Medical Research, Kansas City, Missouri 64110, USA.
Biotech Histochem. 2007 Feb;82(1):23-8. doi: 10.1080/10520290701333558.
Traditionally, cartilage is stained by alcian blue using acidic conditions to differentiate tissue staining. The acidic conditions are problematic when one wishes to stain the same specimen for mineralized bone with alizarin red, because acid demineralizes bone, which negatively affects bone staining. We have developed an acid-free method to stain cartilage and bone simultaneously in zebrafish larvae. This method has the additional advantage that PCR genotyping of stained specimens is possible.
传统上，软骨通过在酸性条件下使用阿尔辛蓝进行染色以区分组织染色。当人们想用茜素红对同一标本的矿化骨进行染色时，酸性条件会带来问题，因为酸会使骨脱矿，这对骨染色产生负面影响。我们开发了一种无酸方法，可同时对斑马鱼幼虫的软骨和骨进行染色。该方法还有一个额外的优点，即对染色标本进行PCR基因分型是可行的。
