Tsuji S, Kanda R
Research Center for Radiation Protection, National Institute of Radiological Sciences, Inage-ku, Chiba, Japan.
Biotech Histochem. 2007 Feb;82(1):29-34. doi: 10.1080/10520290701257153.
We describe here the chemical induction of premature condensed chromosomes in human peripheral lymphocytes after culture for 6 h. Many have attempted this induction without culture or with short-term culture, because this technique permits prompt cytogenetic biodosimetry of radiation accidents. Lymphocytes were separated from blood, incubated in the presence of phytohemagglutinin, ATP, and p34(cdc2)/cyclin B kinase, then treated with calyculin A during the last hour. The culture medium was supplemented with a lower concentration of fetal calf serum than conventionally used to minimize its possible interference with the effects of these drugs. We obtained, rarely, a suitable morphology of premature chromosome condensation in short-term cultured lymphocytes for conventional chromosome aberration analysis.
我们在此描述了在培养6小时后，人类外周血淋巴细胞中早熟凝集染色体的化学诱导方法。许多人曾尝试在无培养或短期培养的情况下进行这种诱导，因为该技术可对辐射事故进行快速的细胞遗传生物剂量测定。从血液中分离淋巴细胞，在植物血凝素、ATP和p34(cdc2)/细胞周期蛋白B激酶存在的情况下进行孵育，然后在最后一小时用花萼海绵诱癌素A处理。培养基中添加的胎牛血清浓度低于常规使用的浓度，以尽量减少其对这些药物作用可能产生的干扰。我们很少能在短期培养的淋巴细胞中获得适合常规染色体畸变分析的早熟染色体凝集形态。
