Sheppeck R A, Garrett K O, Bentz M L, Johnson P C
Department of Surgery, University of Pittsburgh School of Medicine, Pennsylvania.
ASAIO Trans. 1991 Jul-Sep;37(3):M260-1.
The role of von Willebrand's factor (VWF) was examined in platelet deposition on polyethylene by exposing 3/16 inch diameter discs of polyethylene to 111In labeled platelets re-suspended in citrated blood from normal and severe von Willebrand's disease donors. Discs were oscillated for 30 and 60 min, washed, fixed, counted for 111In, and examined by scanning electron microscopy (SEM). Total platelet deposition in the 0% VWF group was significantly greater than control discs at 30 min (7.0 x 10(6) +/- 0.5 compared with 3.29 x 10(6) +/- 0.6 platelets/cm2). The SEM examination revealed patches of spread platelets, with platelets adhering to platelets in controls. A dense, uniform monolayer of platelets was found on the 0% VWF discs. At 60 min, control discs had significant increases in the platelet deposition with SEM examination revealing uniform coverage of the disc with additional platelets.
通过将直径为3/16英寸的聚乙烯圆盘暴露于重悬于来自正常和重度血管性血友病供体的枸橼酸盐血液中的111铟标记血小板，研究了血管性血友病因子（VWF）在血小板在聚乙烯上沉积中的作用。将圆盘振荡30分钟和60分钟，洗涤、固定、计数111铟，并通过扫描电子显微镜（SEM）检查。在30分钟时，0%VWF组的总血小板沉积显著高于对照圆盘（分别为7.0×10⁶±0.5和3.29×10⁶±0.6个血小板/cm²）。SEM检查显示对照组中有铺展的血小板斑块，血小板相互粘附。在0%VWF圆盘上发现了一层密集、均匀的血小板单层。在60分钟时，对照圆盘的血小板沉积显著增加，SEM检查显示圆盘被额外的血小板均匀覆盖。
