Southan C
Global Compound Sciences, AstraZeneca R&D, Mölndal, Sweden.
Biochem Soc Trans. 2007 Jun;35(Pt 3):599-603. doi: 10.1042/BST0350599.
Multiple alignments and phylogenetic tree constructions are established techniques for examining the evolutionary history of protease sequences in organisms such as humans, mice, fruitflies, nematode worms and yeast. They also facilitate the mapping of those conserved positions that are important for structure and catalytic function. However, the continued increase in completed or draft genomes offers new opportunities for examining protease evolution across a broader (e.g. more mammals) and deeper (e.g. more invertebrates) phylogenetic range. In addition, the improving annotation not only of proteases, but also of their substrates, interaction partners in proteolytic complexes and endogenous inhibitor proteins now means that aspects of co-evolution can be addressed. The increasing phylogenetic coverage is also important for resolving orthology issues that arise from protease gene duplication or loss in different lineages. Selected sequences will be used to exemplify the utility of Internet resources and present results for these types of analysis.
多重比对和系统发育树构建是用于研究人类、小鼠、果蝇、线虫和酵母等生物体中蛋白酶序列进化史的既定技术。它们还有助于绘制那些对结构和催化功能至关重要的保守位点。然而，完整基因组或基因组草图数量的持续增加为在更广泛（如更多哺乳动物）和更深层次（如更多无脊椎动物）的系统发育范围内研究蛋白酶进化提供了新机会。此外，不仅蛋白酶的注释不断完善，其底物、蛋白水解复合物中的相互作用伙伴以及内源性抑制剂蛋白的注释也在不断改进，这意味着可以探讨共同进化的各个方面。不断增加的系统发育覆盖范围对于解决因不同谱系中蛋白酶基因复制或丢失而产生的直系同源问题也很重要。将选择序列来说明互联网资源的效用，并展示这些类型分析的结果。
