Benito J M, López M, Lozano S, Ballesteros C, González-Lahoz J, Soriano V
Department of Infectious Diseases, Hospital Carlos III, Madrid, Spain.
Clin Exp Immunol. 2007 Jul;149(1):171-7. doi: 10.1111/j.1365-2249.2007.03412.x. Epub 2007 May 18.
Hydroxyurea (HU) is a cytostatic drug which has been used as an anti-HIV agent due mainly to its synergistic activity when combined with certain anti-retrovirals. In addition, HU might have a beneficial effect on parameters involved in the pathogenesis of HIV infection, such as immune activation. To test this hypothesis, the effect of HU on T cell proliferation and T cell activation, as well as the potential association between these two phenomena, were examined in an in vitro model. HU exerted a dose-dependent anti-proliferative effect on T cells, and modulated the expression of different activation markers. In cells exposed to HU, expression of CD25 and CD38 diminished in a dose-dependent manner, whereas expression of CD69 increased. However, when the expression of these markers was examined separately on proliferating and non-proliferating lymphocytes, HU did not exert any significant effect. Thus, the effect of HU on T cell activation is not direct and seems to be mediated through its effect on T cell proliferation.
羟基脲（HU）是一种细胞生长抑制剂，主要因其与某些抗逆转录病毒药物联合使用时具有协同活性而被用作抗HIV药物。此外，HU可能对HIV感染发病机制中涉及的参数，如免疫激活，具有有益作用。为了验证这一假设，在体外模型中研究了HU对T细胞增殖和T细胞激活的影响，以及这两种现象之间的潜在关联。HU对T细胞发挥剂量依赖性的抗增殖作用，并调节不同激活标志物的表达。在暴露于HU的细胞中，CD25和CD38的表达呈剂量依赖性降低，而CD69的表达增加。然而，当分别在增殖和非增殖淋巴细胞上检测这些标志物的表达时，HU没有发挥任何显著作用。因此，HU对T细胞激活的作用不是直接的，似乎是通过其对T细胞增殖的作用介导的。
