Tian Shen-Peng, Zhang Ji-Hong, Wang Chen-Zhu
State Key Laboratory of Integrated Management of Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, Beijing 100080, China.
J Insect Physiol. 2007 Jul;53(7):699-707. doi: 10.1016/j.jinsphys.2007.03.015. Epub 2007 Apr 10.
Polydnaviruses, symbionts of parasitic ichneumonid (ichnoviruses, IVs) and braconid (bracoviruses, BVs), are injected into hosts along with wasp eggs. Within the host, PDV genes are expressed and their products function to alter lepidopteran host physiology and enable endoparasitoid development. In the present study, we describe two Campoletis chlorideae ichnovirus (CcIV) viral ankyrin (vankyrin) genes and their transcription. The CcIV vankyrin genes possess ankyrin repeat domains that resemble the inhibitory domains of the Drosophila melanogaster NF-kappaB transcription factor inhibitor (IkappaB) cactus. The expression of CcIV vankyrin genes could be detected in Helicoverpa armigera during the whole course of parasitization with two expression peaks, 30 min post-parasitization (p.p.) and 2 days p.p. Our data indicate that the CcIV vankyrin genes are differentially expressed in the tissues of parasitized hosts and both are mainly expressed in hemocytes. The temporal and spatial variation in expression of the two CcIV vankyrin genes suggests that CcIV vankyrin genes could be involved in early protection of parasitoid eggs from host cellular immune response by suppressing NF-kappaB signaling cascades, thereby altering development and immune responses of parasitized lepidopteran hosts.
多分DNA病毒是寄生姬蜂（卵病毒，IVs）和茧蜂（茧蜂病毒，BVs）的共生体，与黄蜂卵一起注入宿主。在宿主体内，多分DNA病毒基因得以表达，其产物发挥作用改变鳞翅目宿主的生理机能，使内寄生蜂得以发育。在本研究中，我们描述了两种氯虫 Campoletis chlorideae 卵病毒（CcIV）的病毒锚蛋白（vankyrin）基因及其转录情况。CcIV病毒锚蛋白基因拥有锚蛋白重复结构域，与果蝇NF-κB转录因子抑制剂（IkappaB）仙人掌的抑制结构域相似。在用氯虫 Campoletis chlorideae 卵病毒寄生棉铃虫的整个过程中，均可检测到CcIV病毒锚蛋白基因的表达，出现两个表达峰值，分别在寄生后30分钟和寄生后2天。我们的数据表明，CcIV病毒锚蛋白基因在被寄生宿主的组织中差异表达，且二者主要在血细胞中表达。两种CcIV病毒锚蛋白基因表达的时空变化表明，CcIV病毒锚蛋白基因可能通过抑制NF-κB信号级联反应，参与早期保护寄生蜂卵免受宿主细胞免疫反应的影响，从而改变被寄生鳞翅目宿主的发育和免疫反应。
