Shi Xizhi, Wu Aibo, Qu Guorun, Li Rongxiu, Zhang Dabing
Shanghai Institutes for Biological Sciences, Ministry of Education, College of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, PR China.
Biomaterials. 2007 Sep;28(25):3741-9. doi: 10.1016/j.biomaterials.2007.04.036. Epub 2007 May 3.
Specific molecularly imprinted polymers (MIPs) for the drug reserpine (RES) using methacrylic acid (MAA) as the functional monomer were developed and characterised for the first time in this study. Evaluation of the various polymers by binding assays indicated that the optimum ratio of functional monomer to template was 4:1. Furthermore, the imprinting effect of the MIPs was assessed by the chromatographic method, which demonstrated that the MIPs had better chromatographic behavior and selectivity than those of the corresponding NIPs. A combination of BET, NMR, UV spectroscopy, and MISPE analyses for investigation of the imprinting and recognition properties revealed that strong specific interactions between the functional monomer and RES in the prepolymerization solutions and the aqueous solutions were probably responsible for RES recognition. The preparation of RES MIPs and elucidation of imprinting and recognition mechanisms may serve as useful references for other drug MIPs.
本研究首次开发并表征了以甲基丙烯酸（MAA）为功能单体的用于药物利血平（RES）的特异性分子印迹聚合物（MIP）。通过结合试验对各种聚合物进行评估表明，功能单体与模板的最佳比例为4:1。此外，通过色谱法评估了MIP的印迹效果，结果表明MIP比相应的非印迹聚合物（NIP）具有更好的色谱行为和选择性。结合BET、NMR、紫外光谱和分子印迹固相萃取（MISPE）分析来研究印迹和识别特性，结果表明预聚合溶液和水溶液中功能单体与RES之间的强特异性相互作用可能是RES识别的原因。RES MIP的制备以及印迹和识别机制的阐明可为其他药物MIP提供有用的参考。
