Facchetti Floriana, Monzani Elena, Cavallini Gabriella, Bergamini Ettore, La Porta Caterina A M
Department of Biomolecular Science and Biotechnology, University of Milan, Italy.
Exp Gerontol. 2007 Jul;42(7):662-7. doi: 10.1016/j.exger.2007.04.001. Epub 2007 Apr 12.
Ageing is accompanied by impaired angiogenesis, as well as by a deficient expression of several angiogenic growth factors and the alteration of endothelial functions. Caloric restriction (CR) is the only intervention that can extend lifespan and retard age-related-decline functions in mammals by reducing the rate of ageing and the progression of the associated diseases. Herein, we have investigated the effects of ageing and of a caloric restriction regimen (mild or severe) on the angiogenic response and on the expression of endothelin-1 (ET-1) in the aorta of male 3-, 12- or 24-month-old Sprague-Dawley rats fed ad libitum (AL), fed ad libitum and fasted 1 day a week (mild CR) or fasted every other in alternate days (severe CR). Our findings, using the rat aorta ring assay, show that the angiogenic capacity of aorta decreases with ageing in the oldest rats only. Furthermore, caloric restriction counteracts the age-related changes caloric restrictions actually give raise to a similar recovery. Interestingly, the mRNA ET-1 levels as well as ET-1 expression in aorta sprouting decreases both in middle and in aged animals. Mild and severe caloric restriction regimens prevents ET-1 changes.
衰老伴随着血管生成受损，以及几种血管生成生长因子的表达不足和内皮功能的改变。热量限制（CR）是唯一一种可以通过降低衰老速度和相关疾病的进展来延长哺乳动物寿命并延缓与年龄相关的功能衰退的干预措施。在此，我们研究了衰老以及热量限制方案（轻度或重度）对雄性3个月、12个月或24个月大的自由摄食（AL）、自由摄食且每周禁食1天（轻度CR）或隔天禁食（重度CR）的Sprague-Dawley大鼠主动脉血管生成反应和内皮素-1（ET-1）表达的影响。我们使用大鼠主动脉环试验的研究结果表明，仅在最年长的大鼠中，主动脉的血管生成能力随衰老而降低。此外，热量限制抵消了与年龄相关的变化，热量限制实际上带来了类似的恢复。有趣的是，中年和老年动物主动脉发芽中的mRNA ET-1水平以及ET-1表达均降低。轻度和重度热量限制方案可防止ET-1变化。
