增殖细胞核抗原（PCNA），复制叉的指挥者。

PCNA, the maestro of the replication fork.

作者信息

Moldovan George-Lucian, Pfander Boris, Jentsch Stefan

机构信息

Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18 82152 Martinsried, Germany.

出版信息

Cell. 2007 May 18;129(4):665-79. doi: 10.1016/j.cell.2007.05.003.

DOI:10.1016/j.cell.2007.05.003

PMID:17512402

Abstract

Inheritance requires genome duplication, reproduction of chromatin and its epigenetic information, mechanisms to ensure genome integrity, and faithful transmission of the information to progeny. Proliferating cell nuclear antigen (PCNA)-a cofactor of DNA polymerases that encircles DNA-orchestrates several of these functions by recruiting crucial players to the replication fork. Remarkably, many factors that are involved in replication-linked processes interact with a particular face of PCNA and through the same interaction domain, indicating that these interactions do not occur simultaneously during replication. Switching of PCNA partners may be triggered by affinity-driven competition, phosphorylation, proteolysis, and modification of PCNA by ubiquitin and SUMO.

摘要

遗传需要基因组复制、染色质及其表观遗传信息的复制、确保基因组完整性的机制，以及将信息准确传递给后代。增殖细胞核抗原（PCNA）——一种环绕DNA的DNA聚合酶辅助因子——通过招募关键因子到复制叉来协调其中的一些功能。值得注意的是，许多参与复制相关过程的因子通过同一相互作用结构域与PCNA的特定面相互作用，这表明这些相互作用在复制过程中不会同时发生。PCNA伙伴的切换可能由亲和力驱动的竞争、磷酸化、蛋白水解以及泛素和类泛素化修饰PCNA所触发。