Moldovan George-Lucian, Pfander Boris, Jentsch Stefan
Department of Molecular Cell Biology, Max Planck Institute of Biochemistry, Am Klopferspitz 18 82152 Martinsried, Germany.
Cell. 2007 May 18;129(4):665-79. doi: 10.1016/j.cell.2007.05.003.
Inheritance requires genome duplication, reproduction of chromatin and its epigenetic information, mechanisms to ensure genome integrity, and faithful transmission of the information to progeny. Proliferating cell nuclear antigen (PCNA)-a cofactor of DNA polymerases that encircles DNA-orchestrates several of these functions by recruiting crucial players to the replication fork. Remarkably, many factors that are involved in replication-linked processes interact with a particular face of PCNA and through the same interaction domain, indicating that these interactions do not occur simultaneously during replication. Switching of PCNA partners may be triggered by affinity-driven competition, phosphorylation, proteolysis, and modification of PCNA by ubiquitin and SUMO.
遗传需要基因组复制、染色质及其表观遗传信息的复制、确保基因组完整性的机制，以及将信息准确传递给后代。增殖细胞核抗原（PCNA）——一种环绕DNA的DNA聚合酶辅助因子——通过招募关键因子到复制叉来协调其中的一些功能。值得注意的是，许多参与复制相关过程的因子通过同一相互作用结构域与PCNA的特定面相互作用，这表明这些相互作用在复制过程中不会同时发生。PCNA伙伴的切换可能由亲和力驱动的竞争、磷酸化、蛋白水解以及泛素和类泛素化修饰PCNA所触发。
