Chiang B Y, Ye C X, Gau X D, Zhou Y, Hong J P, Wang Y S
Department of Cardiothoracic Surgery, Renji Hospital, Shanghai, China.
ASAIO Trans. 1991 Jul-Sep;37(3):M485-7.
To evaluate the effects of pulsatile reperfusion (PR) on the postischemic myocardial phosphometabolites, 17 sheep were put on cardiopulmonary bypass (CPB) and randomly divided into a pulsatile group (P group) and nonpulsatile group (NP group). The heart was arrested by global ischemia for 45 minutes, then defibrillated and reperfused for 2 hours while the circulation was supported by CPB. Myocardial needle biopsies were obtained, and ATP, ADP, and AMP were measured with high performance liquid chromatography. There were no significant differences between the two groups in myocardial ADP and AMP. However, after 120 minutes of reperfusion, the myocardial ATP was restored in the P group, but continued to decrease further in the NP group. Experimental results imply that PR might reduce reperfusion injury and promote recovery of the ischemic myocardium.
为评估搏动性再灌注（PR）对缺血后心肌磷酸代谢物的影响，将17只绵羊置于体外循环（CPB）下，并随机分为搏动组（P组）和非搏动组（NP组）。心脏通过全心缺血停搏45分钟，然后除颤并再灌注2小时，同时由CPB维持循环。获取心肌穿刺活检组织，并用高效液相色谱法测量三磷酸腺苷（ATP）、二磷酸腺苷（ADP）和一磷酸腺苷（AMP）。两组之间心肌ADP和AMP无显著差异。然而，再灌注120分钟后，P组心肌ATP恢复，而NP组心肌ATP继续进一步下降。实验结果表明，PR可能减轻再灌注损伤并促进缺血心肌的恢复。
