急性心肌梗死患者心肌肌钙蛋白T释放及降解情况的研究。
Investigation of release and degradation of cardiac troponin T in patients with acute myocardial infarction.
作者信息
Michielsen Etienne C H J, Diris Jart H C, Kleijnen Vincent W V C, Wodzig Will K W H, Van Dieijen-Visser Marja P
机构信息
Department of Clinical Chemistry, University Hospital Maastricht, P Debyelaan 25, 6229 HX, PO box 5800, NL-6202 AZ, Maastricht, The Netherlands.
出版信息
Clin Biochem. 2007 Aug;40(12):851-5. doi: 10.1016/j.clinbiochem.2007.04.004. Epub 2007 Apr 19.
OBJECTIVES
Cardiac troponin T (cTnT) degradation after tissue release is still under debate. Because degradation of cTnT might have consequences on clearance of the molecule from the circulation, but also on the assay performance, the aim of this study was to investigate cTnT release and degradation in serum of AMI patients.
DESIGN AND METHODS
Serum samples were collected from 20 patients with AMI diagnosis undergoing rapid revascularization. Intact cTnT and fragments were detected using a combination of immunoprecipitation, SDS-PAGE and Western blotting.
RESULTS
The intact cTnT protein was detected only during the first 12 h after the cTnT concentration started to increase above the AMI cut-off value of 0.03 microg/L. Thereafter only fragments with molecular weights ranging from 10 to 30 kDa were detected, with two fragments being most prominent (15 and 25 kDa).
CONCLUSIONS
Intact cTnT rapidly disappears from the circulation during the early hours after AMI, but immunoreactive fragments remain present longer. The current cTnT immunoassay detects both intact cTnT and fragments.
目的
组织释放后心肌肌钙蛋白T（cTnT）的降解仍存在争议。由于cTnT的降解可能不仅会影响该分子从循环中的清除，还会影响检测性能，因此本研究旨在调查急性心肌梗死（AMI）患者血清中cTnT的释放和降解情况。
设计与方法
收集20例诊断为AMI且正在接受快速血运重建的患者的血清样本。使用免疫沉淀、十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和蛋白质免疫印迹法相结合的方法检测完整的cTnT和片段。
结果
仅在cTnT浓度开始升高至高于AMI临界值0.03μg/L后的最初12小时内检测到完整的cTnT蛋白。此后，仅检测到分子量范围为10至30 kDa的片段，其中两个片段最为突出（15和25 kDa）。
结论
AMI后早期，完整的cTnT迅速从循环中消失，但免疫反应性片段存在的时间更长。目前的cTnT免疫测定法可检测完整的cTnT和片段。
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