Berendsen Agnes D, Smit Theo H, Hoeben Kees A, Walboomers X Frank, Bronckers Antonius L J J, Everts Vincent
Department of Oral Cell Biology, Academic Center for Dentistry Amsterdam (ACTA), Universiteit van Amsterdam and Vrije Universiteit, Research Institute MOVE, Amsterdam, The Netherlands.
Biomaterials. 2007 Aug;28(24):3530-6. doi: 10.1016/j.biomaterials.2007.04.031. Epub 2007 May 3.
Reconstruction of tendon and ligament tissues requires proper attachment of the tissue-engineered construct to surrounding tissues. A problem of reconstructing collagen-rich tissues is that an in vitro engineered collagenous network containing fibroblasts will contract and detach from a solid surface. In vivo anchorage of soft connective tissues to mineralized tissues like bones and teeth is accomplished by embedding collagen fibrils into mineralized layers. Mineralization is partially the result of local activity of the enzyme alkaline phosphatase (ALP). In this study, we tested whether ALP-induced mineral deposition at the interface between a collagen gel and a polystyrene or polyetheretherketone (PEEK) surface could prevent gel detachment from the surface. Coating of culture wells with intestinal ALP prevented detachment of gels harbored with human periodontal ligament (PDL) fibroblasts in the presence of its substrate beta-glycerophosphate. Mineral deposition was observed predominantly at the interface of collagen gel and well surface. The contractile properties of fibroblasts were not influenced by either ALP, beta-glycerophosphate or both. The presence of ALP on a solid surface and providing its substrate to allow mineral deposition can prevent detachment of collagen matrices. Our findings provide a tool to induce attachment of fibrillar collagen to a solid surface; an approach that seems useful for reconstruction of load-bearing tissues and attachment of ligaments to implants.
肌腱和韧带组织的重建需要将组织工程构建体正确附着于周围组织。重建富含胶原蛋白的组织存在一个问题，即含有成纤维细胞的体外工程化胶原网络会收缩并从固体表面脱离。体内软结缔组织与骨骼和牙齿等矿化组织的锚固是通过将胶原纤维嵌入矿化层来实现的。矿化部分是碱性磷酸酶（ALP）局部活性的结果。在本研究中，我们测试了ALP诱导的胶原凝胶与聚苯乙烯或聚醚醚酮（PEEK）表面之间界面处的矿物质沉积是否可以防止凝胶从表面脱离。用肠ALP包被培养孔可防止在其底物β-甘油磷酸存在的情况下，含有人类牙周膜（PDL）成纤维细胞的凝胶脱离。矿物质沉积主要在胶原凝胶与孔表面的界面处观察到。成纤维细胞的收缩特性不受ALP、β-甘油磷酸或两者的影响。固体表面存在ALP并提供其底物以允许矿物质沉积可防止胶原基质脱离。我们的研究结果提供了一种诱导纤维状胶原附着于固体表面的工具；这种方法似乎对重建承重组织以及将韧带附着于植入物很有用。
