含芘DNA模拟物的光谱性质。

Spectroscopic properties of pyrene-containing DNA mimics.

作者信息

Samain Florent, Malinovskii Vladimir L, Langenegger Simon M, Häner Robert

机构信息

Department of Chemistry and Biochemistry, University of Bern, Freiestrasse 3, CH-3012 Bern, Switzerland.

出版信息

Bioorg Med Chem. 2008 Jan 1;16(1):27-33. doi: 10.1016/j.bmc.2007.04.052. Epub 2007 May 3.

DOI:10.1016/j.bmc.2007.04.052

PMID:17512737

Abstract

DNA mimics containing non-nucleosidic pyrene building blocks are described. The modified oligomers form stable hybrids, although a slight reduction in hybrid stability is observed in comparison to the unmodified DNA duplex. The nature of the interaction between the pyrene residues in single and double stranded oligomers is analyzed spectroscopically. Intra- and interstrand stacking interactions of pyrenes are monitored by UV-absorbance as well as fluorescence spectroscopy. Excimer formation is observed in both single and double strands. In general, intrastrand excimers show fluorescence emission at shorter wavelengths (approx. 5-10 nm) than excimers formed by interstrand interactions. The existence of two different forms of excimers (intra- vs. interstrand) is also revealed in temperature dependent UV-absorbance spectra.

摘要

描述了含有非核苷芘结构单元的DNA模拟物。修饰后的寡聚物形成稳定的杂交体，尽管与未修饰的DNA双链体相比，杂交体稳定性略有降低。通过光谱分析单链和双链寡聚物中芘残基之间相互作用的性质。利用紫外吸收光谱和荧光光谱监测芘的链内和链间堆积相互作用。在单链和双链中均观察到准分子形成。一般来说，链内准分子的荧光发射波长（约5-10nm）比链间相互作用形成的准分子短。温度依赖性紫外吸收光谱也揭示了两种不同形式准分子（链内与链间）的存在。